Abstract | OBJECTIVE: To evaluate the effect of low-bias flow oscillation (LBFO) with partial liquid ventilation (PLV) on perfluorochemical evaporation, histopathology, and oxidative tissue damage in an animal model of acute lung injury. DESIGN: Prospective, randomized animal study. SETTING: Research laboratory of a health sciences university. SUBJECTS: Twelve New Zealand White rabbits. INTERVENTIONS: Juvenile rabbits were anesthetized, paralyzed, and ventilated through a tracheostomy with either high-frequency oscillatory ventilation or LBFO. Lung injury was induced by repeated saline lavage, after which perflubron was instilled through a side port of the endotracheal tube. Lateral fluoroscopic images were performed at baseline and at various postfill intervals of animals in the high-frequency oscillatory ventilation-PLV and LBFO-PLV groups. The images were digitalized for computer analysis of the Lung Lucency Index, a surrogate marker of perflubron evaporation. Histopathologic evaluation was performed using a lung-injury scoring system. Malondialdehyde was measured in lung homogenates to assess oxidative damage. MEASUREMENTS AND MAIN RESULTS: There were no significant differences in gas exchange and ventilator settings between groups throughout the experiment. At 300 mins, the high-frequency oscillatory ventilation-PLV group had a significantly higher Lung Lucency Index compared with the LBFO-PLV group in both dependent and nondependent lung regions (a high Lung Lucency Index correlates with increased perflubron loss). Malondialdehyde measurements were not different between groups. Animals treated with LBFO-PLV had a lower histopathologic lung-injury score compared with high-frequency oscillatory ventilation-PLV. CONCLUSION: LBFO-PLV is a viable mode of ventilation in a model of acute lung injury and is associated with significant preservation of perflubron in comparison with high-frequency oscillatory ventilation-PLV. The lower evaporative losses during LBFO-PLV were associated with improved histology scores.
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Authors | Budi Wiryawan, Mark S Dowhy, Bradley P Fuhrman, Alexandre T Rotta |
Journal | Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies
(Pediatr Crit Care Med)
Vol. 6
Issue 6
Pg. 690-7
(Nov 2005)
ISSN: 1529-7535 [Print] United States |
PMID | 16276337
(Publication Type: Journal Article)
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Chemical References |
- Fluorocarbons
- Hydrocarbons, Brominated
- perflubron
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Topics |
- Animals
- Disease Models, Animal
- Fluorocarbons
- Fluoroscopy
- High-Frequency Ventilation
(adverse effects)
- Hydrocarbons, Brominated
- Liquid Ventilation
(adverse effects)
- Pulmonary Gas Exchange
- Rabbits
- Random Allocation
- Respiratory Distress Syndrome
(blood, etiology, pathology)
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