Expression of MAGE-D4, a novel MAGE family antigen, is correlated with tumor-cell proliferation of non-small cell lung cancer.

Abstract

PURPOSE:

MAGE-D4, originally termed MAGE-E1, is a novel MAGE family gene that is expressed at high levels in malignant tumors as compared to normal tissue. The present study was conducted to assess the clinical significance of MAGE-D4 expression in non-small cell lung cancer (NSCLC).

EXPERIMENTAL DESIGN:

Expression of MAGE-D4 protein was estimated by immunohistochemistry and MAGE-D4 mRNA expression was investigated using quantitative reverse transcription-PCR (RT-PCR).

RESULTS:

We assessed MAGE-D4 expression in NSCLC tissues and was found to be up-regulated in tumor tissues compared with normal lung tissues (mean MAGE-D4/GAPDH values, 0.035 for tumor tissues and 0.009 for normal lung tissues; p=0.002). However, there was no significant difference in MAGE-D4 expression among different pathological stages. The proliferative activity of tumor cells was significantly higher in high MAGE-D4 tumors (mean proliferative indices, 58.3 for high MAGE-D4 tumor levels and 34.0 for low MAGE-D4 tumor levels; p<0.001). In addition, a high MAGE-D4 expression was more frequently seen in squamous cell carcinoma than in adenocarcinoma (p=0.008), and less frequently in well-differentiated tumors than in moderately to poorly differentiated tumors (p=0.036). There was no difference in the postoperative survival between low and high MAGE-D4 patients (5-year survival rates, 65% and 69%, respectively; p=0.742).

CONCLUSIONS:

MAGE-D4 plays some roles in tumor cells proliferation in NSCLC, but MAGE-D4 expression status did not provided a prognostic significance.

• Authors: Shinya Ito, Yozo Kawano, Hiromichi Katakura, Kazumasa Takenaka, Masashi Adachi, Manabu Sasaki, Keiji Shimizu, Kazuhiro Ikenaka, Hiromi Wada, Fumihiro Tanaka
• Journal: Lung cancer (Amsterdam, Netherlands) (Lung Cancer) Vol. 51 Issue 1 Pg. 79-88 (Jan 2006) ISSN: 0169-5002 [Print] Ireland
• PMID: 16225959 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• MAGED1 protein, human
• Neoplasm Proteins
• RNA, Messenger

Topics

• Animals
• Antigens, Neoplasm
• Apoptosis
• Carcinoma, Non-Small-Cell Lung (immunology, metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Immunoblotting
• Intracellular Fluid (metabolism)
• Lung Neoplasms (immunology, metabolism, pathology)
• Mice
• Mice, Inbred BALB C
• Microscopy, Fluorescence
• Neoplasm Proteins (genetics, immunology, metabolism)
• Polymerase Chain Reaction
• Prognosis
• RNA, Messenger (genetics)