Abstract |
Adaptation of the skin to repeated influence of exogenous irritants is called the hardening phenomenon. We investigated the stratum corneum lipid composition before and after induction of a hardening phenomenon. Irritant contact dermatitis was induced in 23 non-atopic volunteers by repeated occlusive application of 0.5% sodium lauryl sulfate (SLS) over 3 weeks. At 3, 6 and 9 weeks after irritation, the SLS responses of pre-irritated skin and normal skin were compared. The horny layer lipid composition ( ceramides 1-7, cholesterol and free fatty acids) was assessed before irritation and 3, 6 and 9 weeks after irritation. During the first 2 weeks of irritation the transepidermal water loss increased continuously and seemed to decrease during the third week (effect of adaptation). The barrier function of pre-irritated sites was more stable to SLS challenge. Three weeks after irritation, there was a significant increase of ceramide 1 (p<0.001). The only volunteer without hardening phenomenon showed no increase of ceramide 1. Ceramide 1 seems to play a key role as a protection mechanism against repeated irritation.
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Authors | Christian Heinemann, Christiane Paschold, Joachim Fluhr, Walter Wigger-Alberti, Sibylle Schliemann-Willers, Hany Farwanah, Klaus Raith, Reinhard Neubert, Peter Elsner |
Journal | Acta dermato-venereologica
(Acta Derm Venereol)
Vol. 85
Issue 4
Pg. 290-5
( 2005)
ISSN: 0001-5555 [Print] Sweden |
PMID | 16191847
(Publication Type: Evaluation Study, Journal Article)
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Chemical References |
- Ceramides
- Fatty Acids
- Irritants
- Sodium Dodecyl Sulfate
- Cholesterol
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Topics |
- Administration, Cutaneous
- Adolescent
- Adult
- Case-Control Studies
- Ceramides
(metabolism)
- Cholesterol
(metabolism)
- Chromatography, High Pressure Liquid
- Dermatitis, Irritant
(etiology, metabolism, pathology)
- Fatty Acids
(metabolism)
- Female
- Humans
- Irritants
(administration & dosage, pharmacology)
- Lipid Metabolism
- Male
- Skin
(drug effects, metabolism, pathology)
- Sodium Dodecyl Sulfate
(administration & dosage, pharmacology)
- Water Loss, Insensible
(drug effects)
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