Abstract | OBJECTIVE: METHODS: Total RNA was extracted from normal adrenal medulla and pheochromocytomas. The expression of ADM and RAMP2/CRLR mRNA were studied by reverse transcription-polymerase chain reaction. The ratios of ADM/GAPDH, RAMP2/ GAPDH, CRLR/GAPDH were used to evaluate the expression levels of ADM, RAMP2 and CRLR mRNA. RESULTS: Expressions of ADM and its receptor- RAMP2/CRLR mRNA were detected in normal adrenal medulla and pheochromocytoma tissues. ADM/GAPDH were 0.48+/-0.09 and 0.75+/-0.24, RAMP2/ GAPDH 0.79+/-0.12 and 1.29+/-0.30, CRLR/GAPDH 0.40+/-0.08 and 0.87+/-0.22 in normal adrenal medulla and pheochromocytomas, respectively (P < 0.05). CONCLUSION: ADM exerts a possible autocrine or paracrine effect in the adrenal. ADM may be involved in the pathogenesis of pheochromocytoma.
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Authors | Dong-Mei Liu, Zheng-Pei Zeng, Han-Zhong Li, Xin-Rong Fan, Guo-Qiang Liu, Wei-Gang Yan, An-Li Tong, Xin Zheng |
Journal | Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
(Zhongguo Yi Xue Ke Xue Yuan Xue Bao)
Vol. 27
Issue 4
Pg. 452-6
(Aug 2005)
ISSN: 1000-503X [Print] China |
PMID | 16178438
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Intracellular Signaling Peptides and Proteins
- Membrane Proteins
- Peptides
- RAMP2 protein, human
- RNA, Messenger
- Receptor Activity-Modifying Protein 2
- Receptor Activity-Modifying Proteins
- Receptors, Adrenomedullin
- Receptors, Calcitonin
- Receptors, Peptide
- Adrenomedullin
- Calcitonin Gene-Related Peptide
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Topics |
- Adrenal Gland Neoplasms
(metabolism)
- Adrenal Medulla
(metabolism)
- Adrenomedullin
- Adult
- Calcitonin Gene-Related Peptide
(biosynthesis, genetics)
- Female
- Humans
- Intracellular Signaling Peptides and Proteins
(genetics)
- Male
- Membrane Proteins
(biosynthesis, genetics)
- Middle Aged
- Peptides
(genetics, metabolism)
- Pheochromocytoma
(metabolism)
- RNA, Messenger
(biosynthesis, genetics)
- Receptor Activity-Modifying Protein 2
- Receptor Activity-Modifying Proteins
- Receptors, Adrenomedullin
- Receptors, Calcitonin
(biosynthesis, genetics)
- Receptors, Peptide
(metabolism)
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