Effects of Tempol, a membrane-permeable radical scavenger, in a rodent model periodontitis.

Abstract

BACKGROUND:

Recent studies have demonstrated that Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl), a cell membrane-permeable radical scavenger, exerts protective effects in various models of inflammation and shock. Reactive oxygen species (ROS) plays a pivotal role in the induction of genes involved in physiological processes as well as in the response to inflammation.

AIM:

We have investigated the effect of Tempol in a rat model of periodontitis.

MATERIALS AND METHODS:

Periodontitis was induced in rats by placing a 2/0 braided silk ligature around the lower left first molar. At day 8, the gingivomucosal tissue encircling the mandibular first molar was removed for evaluation of neutrophils infiltration, tissue permeability, nitrotyrosine formation, poly-(ADP-ribose) polymerase (PARP) activation, radiography and histology.

RESULTS AND CONCLUSIONS:

Legation significantly induced an increased neutrophil infiltration and a positive staining for nitrotyrosine formation and PARP activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone erosion as evaluated by radiography analysis. Intraperitonial injection of Tempol (10 mg/kg daily for 8 days) significantly decreased all of the parameters of inflammation as described above. This suggests that antioxidant therapies, which interfere with ROS, may be of benefit in the treatment of periodontitis.

• Authors: Rosanna Di Paola, Emanuela Mazzon, Daniele Zito, Daniele Maiere, Domenico Britti, Tiziana Genovese, Salvatore Cuzzocrea
• Journal: Journal of clinical periodontology (J Clin Periodontol) Vol. 32 Issue 10 Pg. 1062-8 (Oct 2005) ISSN: 0303-6979 [Print] United States
• PMID: 16174269 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyclic N-Oxides
• Free Radical Scavengers
• Spin Labels
• 3-nitrotyrosine
• Tyrosine
• Poly(ADP-ribose) Polymerases
• tempol

Topics

• Alveolar Bone Loss (drug therapy)
• Animals
• Cyclic N-Oxides (therapeutic use)
• Drug Evaluation, Preclinical
• Free Radical Scavengers (therapeutic use)
• Male
• Mandibular Diseases (drug therapy)
• Models, Animal
• Periodontitis (drug therapy, enzymology)
• Poly(ADP-ribose) Polymerases (drug effects)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Spin Labels
• Tyrosine (analogs & derivatives, drug effects)