Induction of apoptotic cell death by 2'-hydroxycinnamaldehyde is involved with ERK-dependent inactivation of NF-kappaB in TNF-alpha-treated SW620 colon cancer cells.

Abstract

2'-Hydroxycinnamaldehyde (HCA) inhibits cell growth of several human cancer cells with unknown mechanisms. We investigated the inhibitory effect of HCA on TNF-alpha-induced cell growth and possible signal pathway in SW620 colon cancer cells. HCA inhibited TNF-alpha-induced SW620 colon cell growth in time- and dose-dependent manner through induction of apoptotic cell death. Parallel with inhibitory effect on cell growth, HCA dose dependency inhibited TNF-alpha-induced activation of NF-kappaB accompanied with inhibition of the translocation of p50. HCA also induced expression of caspase-3 and Bax, but decreased Bcl-2. HCA furthermore activated ERK pathway, and ERK inhibitor reversed inhibitory effect of HCA on cell growth and transcriptional activation of NF-kappaB. These results demonstrate that HCA inhibits cell growth through induction of apoptotic cell death by ERK pathway-dependent NF-kappaB inactivation.

• Authors: Seung Ho Lee, Chung Woo Lee, Jae Woong Lee, Myoung Suk Choi, Dong Ju Son, Youn Bok Chung, Chong Kil Lee, Ki Wan Oh, Dong Chul Moon, Byoung Mog Kwon, Jin Tae Hong
• Journal: Biochemical pharmacology (Biochem Pharmacol) Vol. 70 Issue 8 Pg. 1147-57 (Oct 15 2005) ISSN: 0006-2952 [Print] England
• PMID: 16143316 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cinnamates
• NF-kappa B
• Tumor Necrosis Factor-alpha
• p-hydroxycinnamaldehyde
• Extracellular Signal-Regulated MAP Kinases

Topics

• Apoptosis (drug effects)
• Blotting, Western
• Cell Line, Tumor
• Cinnamates (pharmacology)
• Colonic Neoplasms (enzymology, pathology)
• Electrophoretic Mobility Shift Assay
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Humans
• NF-kappa B (antagonists & inhibitors)
• Tumor Necrosis Factor-alpha (pharmacology)