Surface and soluble triggering receptor expressed on myeloid cells-1: expression patterns in murine sepsis.

Abstract

OBJECTIVE:

To examine the expression patterns of the triggering receptor expressed on myeloid cells (TREM)-1 during experimental septic shock.

DESIGN:

Animal study.

SETTING:

Animal research laboratory.

SUBJECTS:

Male BALB/c mice, 7-9 wks of age.

INTERVENTIONS:

Septic shock was induced by cecal ligation and puncture in eight mice. Eight additional animals were sham-operated and served as a control group. All animals were resuscitated by fluid infusion and administered antibiotics. Kill was performed under anesthesia 12, 24, or 48 hrs later.

MEASUREMENTS AND MAIN RESULTS:

Surface expression of TREM-1 was analyzed using flow cytometry on peripheral blood cells, peritoneal macrophages and neutrophils, splenic macrophages, and Kupffer cells. Gene expression was also studied in these same cells using reverse transcription-polymerase chain reaction. Tumor necrosis factor-alpha, interleukin-1beta, and soluble TREM-1 concentrations were determined in plasma and peritoneal lavage fluid. Sepsis strongly induced TREM-1 gene expression, which translated into an up-regulation of TREM-1 surface expression on neutrophils and monocytes/macrophages both at the focus on infection as well as distally. Moreover, sepsis induced the release of significant levels of soluble TREM-1. Plasma soluble TREM-1 concentrations negatively correlated with tumor necrosis factor-alpha and interleukin-1beta levels at 12 hrs.

CONCLUSIONS:

These results provide new information as to the regulation of TREM-1 during sepsis. Considering that both cell-surface and soluble TREM-1 were strongly up-regulated during infection, this study may add support to the putative usefulness of TREM-1 as a diagnostic tool.

• Authors: Sébastien Gibot, Frédéric Massin, Pol Le Renard, Marie C Béné, Gilbert C Faure, Pierre-Edouard Bollaert, Bruno Levy
• Journal: Critical care medicine (Crit Care Med) Vol. 33 Issue 8 Pg. 1787-93 (Aug 2005) ISSN: 0090-3493 [Print] United States
• PMID: 16096457 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Membrane Glycoproteins
• Receptors, Immunologic
• TREM1 protein, human
• Triggering Receptor Expressed on Myeloid Cells-1

Topics

• Animals
• Male
• Membrane Glycoproteins (metabolism)
• Mice
• Mice, Inbred BALB C
• Receptors, Immunologic (metabolism)
• Shock, Septic (immunology)
• Statistics, Nonparametric
• Triggering Receptor Expressed on Myeloid Cells-1
• Up-Regulation