[Convenient assay for glycated apolipoprotein B using protease].

Abstract

The aim of this study is to develop a convenient method for monitoring glycated apolipoprotein B levels. Serum sample was treated with dextran-magnesium and the resulting precipitates were subjected to glycated albumin assay. Dissolving the precipitates by Triton X-100 and digesting by proteinase K enable the establishment of stable and sensitive assay. Intra- and inter assay coefficients of variation were 1.5-3.5% and 1.6-3.3%, respectively. The serum glycated apolipoprotein B values by present method correlated well with those by enzyme-linked immunosorbent assay (r=0.979). The serum glycated apolipoprotein B values in healthy subjects was 4.14+/-0.51% (mean+/-SD) with no significant difference between men and women and with no age-dependent variation. Patients with type 2 diabetes mellitus had higher serum glycated apolipoprotein B levels than the healthy subjects. This assay should further be investigated to establish the validity of glycated apolipoprotein B measurement in clinical field.

• Authors: Koji Shishino, Masaaki Ochi, Mitsuharu Murase, Haruhiko Osawa, Hideichi Makino
• Journal: Rinsho byori. The Japanese journal of clinical pathology (Rinsho Byori) Vol. 53 Issue 6 Pg. 504-8 (Jun 2005) ISSN: 0047-1860 [Print] Japan
• PMID: 16026076 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Glycation End Products, Advanced
• Lipoproteins, LDL
• glycated lipoproteins, LDL
• Endopeptidase K

Topics

• Adult
• Biomarkers (blood)
• Diabetes Mellitus, Type 2 (diagnosis)
• Endopeptidase K
• Female
• Glycation End Products, Advanced
• Humans
• Lipoproteins, LDL (blood)
• Male
• Middle Aged
• Reference Values