Abstract | OBJECTIVES: The secreted form of ADAM12 is a metalloprotease that may be involved in placental and fetal growth. We examined whether the concentration of ADAM12 in first-trimester maternal serum could be used as a marker for preeclampsia. METHODS: We developed a semiautomated, time-resolved, immunofluorometric assay for the quantification of ADAM12 in serum. The assay detected ADAM12 in a range of 78-1248 microg/L. Serum samples derived from women in the first trimester of a normal pregnancy (n = 324) and from women who later developed preeclampsia during pregnancy (n = 160) were obtained from the First Trimester Copenhagen Study. ADAM12 levels were assayed in these serum samples. Serum levels of ADAM12 were converted to multiples of the median (MoM) after log-linear regression of concentration versus gestational age. RESULTS: Serum ADAM12 levels in women who developed preeclampsia during pregnancy had a mean log MoM of -0.066, which was significantly lower than the mean log MoM of -0.001 for ADAM12 levels observed in serum samples from women with normal pregnancy (P = .008). The mean log MoM was even lower in serum derived from preeclamptic women whose infant's weight at birth was less than 2,500 g (n = 27, mean log MoM of -0.120, P = .053). CONCLUSION: LEVEL OF EVIDENCE: II-2.
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Authors | Jennie Laigaard, Tina Sørensen, Sophie Placing, Peter Holck, Camilla Fröhlich, Karen R Wøjdemann, Karin Sundberg, Anne-Cathrine Shalmi, Ann Tabor, Bent Nørgaard-Pedersen, Bent Ottesen, Michael Christiansen, Ulla M Wewer |
Journal | Obstetrics and gynecology
(Obstet Gynecol)
Vol. 106
Issue 1
Pg. 144-9
(Jul 2005)
ISSN: 0029-7844 [Print] United States |
PMID | 15994630
(Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Biomarkers
- Disintegrins
- Metalloproteases
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Topics |
- Adult
- Biomarkers
(blood)
- Case-Control Studies
- Disintegrins
(blood)
- Female
- Fluorescent Antibody Technique
(methods)
- Humans
- Metalloproteases
(blood)
- Pre-Eclampsia
(diagnosis, prevention & control)
- Predictive Value of Tests
- Pregnancy
- Pregnancy Trimester, First
- Probability
- Reference Values
- Risk Factors
- Sampling Studies
- Sensitivity and Specificity
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