Antineutrophil cytoplasmic antibodies (ANCA) in Chinese patients with anti-GBM crescentic glomerulonephritis.

Abstract

OBJECTIVE:

To study the prevalence of ANCA and their target antigen in Chinese patients with anti-GBM crescentic glomerulonephritis (CGN), and to evaluate the possible role of ANCA in Chinese anti-GBM CGN patients with coexisting serum ANCA by studying clinicopathologic features of this disease.

MATERIAL AND METHODS:

Twenty-three sera were collected from 23 renal biopsy-proven anti-GBM CGN patients. According to the standardized procedures, all of the sera were determined by both, indirect immunofluorescence (IIF) ANCA, and enzyme-linked immunosorbent assay (ELISA) MPO-ANCA, PR3-ANCA and BPI-ANCA. The patients were divided into two groups according to serum ANCA positivity (Group A) or negativity (Group B). Thirty-three ANCA-associated pauci-immune CGN patients were regarded as control group (Group C). Their clinicopathologic features were compared to reveal whether ANCA correlated with disease activity.

RESULTS:

There were 11 (47.8%) cases with positive serum ANCA in 23 anti-GBM glomerulonephritis patients. There were 4/11 MPO-ANCA (one with positive PR3-ANCA and C-ANCA, three with negative IIF-ANCA), 1/11 PR3-ANCA (with positive MPO-ANCA and C-ANCA), 3/11 P-ANCA (with negative ELISA-ANCA) and 5/11 C-ANCA (one with positive PR3-ANCA and MPO-ANCA, and the other four with negative ELISA-ANCA). No BPI-ANCA was detected. No different clinicopathologic features were found between Groups A and B. Both were different from Group C in age, sex ratio, frequence of anuria and ESRD, variety of crescents, glomerular sclerosis, vessel lesion and prognosis.

CONCLUSION:

Our data demonstrate that ANCA in Chinese patients with anti-GBM CGN is not rare. The major target antigen of ANCA is MPO. ANCA seems not to be correlated with disease activity.

• Authors: G Yang, Z Tang, Y Chen, C Zeng, H Chen, Z Liu, L Li
• Journal: Clinical nephrology (Clin Nephrol) Vol. 63 Issue 6 Pg. 423-8 (Jun 2005) ISSN: 0301-0430 [Print] Germany
• PMID: 15960143 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antibodies, Antineutrophil Cytoplasmic
• Biomarkers

Topics

• Adolescent
• Adult
• Aged
• Anti-Glomerular Basement Membrane Disease (blood, epidemiology, immunology)
• Antibodies, Antineutrophil Cytoplasmic (blood)
• Biomarkers (blood)
• Biopsy
• China (epidemiology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Follow-Up Studies
• Humans
• Kidney (pathology)
• Male
• Middle Aged
• Prevalence
• Retrospective Studies
• Severity of Illness Index