Protection induced by vaccinating sheep with a thiol-binding extract of Haemonchus contortus membranes is associated with its protease components.

Abstract

Previous work has shown that a protein extract enriched for cysteine protease activity (TSBP) prepared from adult Haemonchus contortus using thiol sepharose affinity chromatography confers substantial protection against a single challenge infection. TSBP comprised proteases and other proteins. Here, TSBP were further fractionated using anion-exchange chromatography and fractions pooled on the basis of containing (1) protease activity, (2) a prominent non-protease peptide and (3) material which did not bind to the column. A protection trial showed that only the protease-enriched material conferred protective immunity and this was comparable to that observed in a TSBP-immunized positive control group. Immunization stimulated a marked IgG response with the IgG2 isotype predominating.

• Authors: D P Knox, S K Smith, D L Redmond, W D Smith
• Journal: Parasite immunology (Parasite Immunol) Vol. 27 Issue 4 Pg. 121-6 (Apr 2005) ISSN: 0141-9838 [Print] England
• PMID: 15910420 (Publication Type: Clinical Trial, Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Helminth
• Antigens, Helminth
• Helminth Proteins
• Immunoglobulin G
• Vaccines, Subunit
• Cysteine Endopeptidases

Topics

• Animals
• Antibodies, Helminth (blood)
• Antigens, Helminth (chemistry, immunology, isolation & purification)
• Cysteine Endopeptidases (immunology, isolation & purification)
• Enzyme-Linked Immunosorbent Assay
• Feces (parasitology)
• Haemonchiasis (immunology, prevention & control, veterinary)
• Haemonchus (immunology)
• Helminth Proteins (immunology, isolation & purification)
• Immunoglobulin G (blood)
• Parasite Egg Count
• Sheep
• Sheep Diseases (immunology, prevention & control)
• Vaccination (veterinary)
• Vaccines, Subunit (chemistry, immunology)