Enhancing of measles virus infection by magnetofection.

Abstract

Magnetofection is a viral and non-viral gene delivery method using polyethyleneimine-conjugated super-paramagnetic nanoparticle under a magnetic field. Previous studies have indicated that magnetofection enhanced the infection of adenoviruses and retroviruses. It is shown that magnetofection enhances the infection of measles virus, a paramyxovirus. When cells expressing a measles virus receptor human SLAM were infected with a measles virus that encodes green fluorescent protein gene, magnetofection enhanced measles virus infection by 30- to 70-fold. The infection of SLAM-negative cells with measles virus was also enhanced by magnetofection, but to a lesser extent. These results indicate that magnetofection could be useful for isolation of measles virus from clinical specimens.

• Authors: Shin-ichi Kadota, Tetsuya Kanayama, Naoko Miyajima, Kaoru Takeuchi, Kyosuke Nagata
• Journal: Journal of virological methods (J Virol Methods) Vol. 128 Issue 1-2 Pg. 61-6 (Sep 2005) ISSN: 0166-0934 [Print] Netherlands
• PMID: 15904981 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• Glycoproteins
• Immunoglobulins
• Receptors, Cell Surface
• Green Fluorescent Proteins
• Signaling Lymphocytic Activation Molecule Family Member 1

Topics

• Animals
• Antigens, CD
• CHO Cells (virology)
• Cell Line
• Chlorocebus aethiops
• Cricetinae
• Glycoproteins (genetics, metabolism)
• Green Fluorescent Proteins (genetics, metabolism)
• HeLa Cells (virology)
• Humans
• Immunoglobulins (genetics, metabolism)
• Magnetics
• Measles virus (genetics, metabolism, pathogenicity)
• Receptors, Cell Surface
• Signaling Lymphocytic Activation Molecule Family Member 1
• Vero Cells (virology)