Abstract | OBJECTIVE: METHODS: RESULTS: PBL of patients with MPO- ANCA-associated vasculitis produced MPO- ANCA following Con-A stimulation, and this effect was inhibited by treatment with cyclosporin A (CyA) or elimination of CD4 cells. PCR-SSCP showed autoantigen-reactive oligoclonal T-cell accumulation in PBMC of one of these patients. We established MPO-specific TCL which secreted interferon-gamma (IFN-gamma), but not interleukin-4 (IL-4); all TCL were CD4 positive, CD8 negative, and HLA-DR restricted. CONCLUSIONS: Our results suggest that Th1-type T cells may mediate MPO- ANCA production, and may play a role in the onset of MPO- ANCA vasculitis.
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Authors | M Yoshida, T Iwahori, I Nakabayashi, M Akashi, T Watanabe, N Yoshikawa |
Journal | Clinical and experimental rheumatology
(Clin Exp Rheumatol)
2005 Mar-Apr
Vol. 23
Issue 2
Pg. 227-30
ISSN: 0392-856X [Print] Italy |
PMID | 15895894
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Antineutrophil Cytoplasmic
- Autoantigens
- Mitogens
- Peroxidase
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Topics |
- Aged
- Antibodies, Antineutrophil Cytoplasmic
(immunology)
- Autoantigens
(immunology)
- Cell Culture Techniques
(methods)
- Female
- Flow Cytometry
- Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
(genetics)
- Humans
- Male
- Middle Aged
- Mitogens
(pharmacology)
- Peroxidase
(immunology)
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Th1 Cells
(drug effects, immunology, pathology)
- Vasculitis, Leukocytoclastic, Cutaneous
(enzymology, immunology, pathology)
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