Cytotoxicity determination without photochemical artifacts.

Abstract

A study was performed to improve cytotoxicity determinations by eliminating flavin-mediated photosensitization from tests with KB cells, NCI-H69 cells, P-glycoprotein expressing KBC5-8 cells, MRP1-expressing H69AR cells, and A240286S human lung adenocarcinoma cells. Growth inhibition by cis-platin, doxorubicin, etoposide, gemcitabine, taxol, vincristine, vinblastine, and vinorelbine was determined under flavin-protecting conditions using flavin-free culture media with fetal bovine serum as the only source of flavins. As compared to conventional tests, the IC50 values determined under flavin-protecting conditions reflected increased apparent drug cytotoxicities, and were flawlessly reproducible. Flavin-mediated photosensitization should, therefore, be strictly eliminated from in vitro experiments involving cytotoxic and other drugs.

• Authors: Michael Heuser, Marijana Kopun, Werner Rittgen, Christof Granzow
• Journal: Cancer letters (Cancer Lett) Vol. 223 Issue 1 Pg. 57-66 (Jun 01 2005) ISSN: 0304-3835 [Print] Ireland
• PMID: 15890237 (Publication Type: Journal Article)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Topoisomerase II Inhibitors
• Riboflavin

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 1 (analysis)
• Cell Proliferation (drug effects)
• Drug Resistance, Multiple
• Drug Resistance, Neoplasm
• Drug Screening Assays, Antitumor (methods)
• Humans
• KB Cells
• Photochemistry
• Riboflavin (chemistry)
• Topoisomerase II Inhibitors