Abstract | OBJECTIVE AND DESIGN: To investigate the role of inflammatory mediators in the hepatoprotective effect of glycine against lipopolysaccharide (LPS)-induced liver injury in rats. MATERIAL OR SUBJECTS: Male Wistar rats were used (N = 4 or 5 per group). Precision-cut liver slices (PCLS) were prepared for in vitro studies. TREATMENT:
Glycine (10 mM) and LPS (10 mug/ml) were added to the incubation medium of PCLS obtained 3 h after LPS intraperitoneal (i. p.) administration (10 mg/kg) or saline injection to rats. Glycine effects were also investigated in vivo by treating rats with a diet containing glycine (5%) during 3 days. METHODS: RESULTS:
Glycine added to the culture medium increased both ATP and glycogen contents of PCLS from LPS-treated rats, reduced the production of TNF-alpha and NOx whereas PGE(2) secretion by PCLS increased. In contrast to the in vitro effect of glycine, we observed that a glycine-enriched diet decreased PGE(2) secretion in the serum after LPS challenge. CONCLUSION: The effect of glycine on LPS-induced mediator secretion is different considering in vitro or in vivo situations. Interestingly, glycine in vitro is able to prevent energy status depletion of PCLS occurring upon inflammation, a phenomenon probably linked to change in inflammatory mediator secretion pattern by hepatic immune cells, namely Kupffer cells.
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Authors | A M Neyrinck, S Margagliotti, N M Delzenne |
Journal | Inflammation research : official journal of the European Histamine Research Society ... [et al.]
(Inflamm Res)
Vol. 54
Issue 3
Pg. 106-12
(Mar 2005)
ISSN: 1023-3830 [Print] Switzerland |
PMID | 15883743
(Publication Type: Journal Article)
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Chemical References |
- Culture Media
- Free Radicals
- Lipopolysaccharides
- Tumor Necrosis Factor-alpha
- Nitric Oxide
- Adenosine Triphosphate
- Glycogen
- L-Lactate Dehydrogenase
- Aspartate Aminotransferases
- Alanine Transaminase
- Dinoprostone
- Oxygen
- Glycine
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Topics |
- Adenosine Triphosphate
(chemistry)
- Alanine Transaminase
(metabolism)
- Analysis of Variance
- Animals
- Aspartate Aminotransferases
(metabolism)
- Culture Media
(pharmacology)
- Dinoprostone
(metabolism)
- Free Radicals
(chemistry)
- Glycine
(chemistry, pharmacology)
- Glycogen
(chemistry)
- Inflammation
- Kupffer Cells
(metabolism)
- L-Lactate Dehydrogenase
(metabolism)
- Lipopolysaccharides
(pharmacology)
- Liver
(drug effects, metabolism)
- Male
- Nitric Oxide
(chemistry)
- Oxygen
(chemistry)
- Rats
- Rats, Wistar
- Tumor Necrosis Factor-alpha
(metabolism)
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