Primary structure of dog preproendothelin-3 and elevated gene expression in kidney affected with interstitial nephritis.

Abstract

To compare the structure of the precursor polypeptide of dog endothelin-3, preproendothelin-3 (PPET-3), with the PPET-3 of other mammals, we cloned dog cDNA from lung tissue using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. An open reading frame encoding a 198-amino-acid polypeptide was found in the cDNA. Regions corresponding to a bioactive mature endothelin-3 peptide, an intermediate form known as big-endothelin-3 and an endothelin-3- like peptide were observed in the putative PPET-3. Comparative analysis showed that the similarity of the dog open reading frame sequence with those from human hypothalamus, mouse intestine, and rat eye is 76.2%, 69.5% and 66.3%, respectively, and that the similarity at the amino acid level is 65.6%, 59.8% and 58.8%, respectively. RT-PCR demonstrated significant elevated expression of PPET-3 mRNA in the kidney of dog with interstitial nephritis.

• Authors: Yuki Fujimori, Tsuyoshi Uchide, Kyosuke Temma, Takushi Sasaki, Keiichiro Kizaki, Yukio Hara, Satoshi Takizawa, Kaname Saida
• Journal: Journal of cardiovascular pharmacology (J Cardiovasc Pharmacol) Vol. 44 Suppl 1 Pg. S426-9 (Nov 2004) ISSN: 1533-4023 [Electronic] United States
• PMID: 15838339 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Endothelin-3
• Protein Precursors
• RNA, Messenger

Topics

• Amino Acid Sequence
• Animals
• Cloning, Molecular
• Disease Models, Animal
• Dogs
• Endothelin-3 (chemistry, genetics)
• Humans
• Kidney (chemistry)
• Mice
• Molecular Sequence Data
• Nephritis, Interstitial (genetics, metabolism)
• Open Reading Frames
• Protein Precursors (chemistry, genetics)
• RNA, Messenger (analysis)
• Rats
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Alignment
• Sequence Analysis, Protein
• Sequence Homology, Amino Acid
• Up-Regulation