Cholesterol as target of Fe-NTA-induced lipid peroxidation in rat tissues.

Abstract

Intraperitoneal injection of the iron-chelate, ferric-nitrilotriacetate (Fe-NTA), induces renal proximal tubular damage associated with oxidative damage in vivo. A sub-lethal dose of Fe-NTA (15 mg Fe/kg body weight) was administered IP to rats; animals were sacrificed and liver, kidney and plasma were collected 1-4 h after injection. In response to the Fe-NTA administration, there were significant time-dependent reductions of the levels of total lipids, cholesterol and total unsaturated fatty acids, and a rise in the concentrations of conjugated dienes, 7-ketocholesterol and fatty acids hydroperoxides, showing a pattern inversely correlated in plasma, kidney and liver. Cholesterol level decreased significantly from 1 h after injection in the kidney and 3-4 h in the plasma and liver of treated rats. This is the first report on cholesterol reduction and accumulated 7-ketocholesterol in the tissues of rats treated with Fe-NTA as a consequence of lipid peroxidation.

• Authors: Antonella Rosa, Monica Deiana, Giulia Corona, Angela Atzeri, Alessandra Incani, M Assunta Dessì
• Journal: Toxicology letters (Toxicol Lett) Vol. 157 Issue 1 Pg. 1-8 (May 16 2005) ISSN: 0378-4274 [Print] Netherlands
• PMID: 15795088 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carcinogens
• Ferric Compounds
• Cholesterol
• Nitrilotriacetic Acid
• ferric nitrilotriacetate

Topics

• Animals
• Carcinogens (toxicity)
• Cholesterol (metabolism)
• Ferric Compounds (toxicity)
• Infusions, Parenteral
• Kidney (chemistry, pathology)
• Lipid Peroxidation
• Liver (chemistry, pathology)
• Male
• Nitrilotriacetic Acid (analogs & derivatives, toxicity)
• Oxidative Stress
• Rats
• Rats, Wistar