Mongolian gerbils are an ideal animal model to explore the role of H. pylori on
cancer development. However, there have been no established
adenocarcinoma cell lines from this model animal. In the present study, we have established
cancer cell lines from a primary
gastric cancer tissue of a Mongolian gerbil. The derived cells could be stably attached with H. pylori, revealed under a scanning electron microscope, and easily transplanted to the nude mice. Rapid phosphorylation of IkappaB, Erk1/2, and AKT of these cells was observed by
Interleukin-1 beta stimulation, and
luciferase reporter gene assay on transcriptional activation of
Nuclear Factor kappa B after challenging with either H. pylori NCTC11637 or its isogenic cagE-knockout mutant, H. pylori revealed the cagE-dependent
NF-kappaB transcriptional activation. The newly established
cancer cell lines from the in vivo gastric
carcinogenesis model animal, the Mongolian gerbil, can be used to develop effective therapeutic strategies against
gastric cancer, especially in exploring the effect of H. pylori, and thus might greatly contribute to
gastric cancer prevention and treatment in humans.