Serological diagnosis of infectious mononucleosis by chemiluminescent immunoassay using capsid antigen p18 of Epstein-Barr virus.

Abstract

BACKGROUND:

Infectious mononucleosis is the common clinical manifestation of primary Epstein-Barr virus (EBV) infection in young children. We evaluated a chemiluminescent immunoassay method for the determination of serum anti-viral capsid antigen IgM antibody and its clinical value in the diagnosis of infectious mononucleosis.

METHODS:

Concentrations of the antibody in serum samples from 187 children measured by chemiluminescent immunoassay were compared with those measured by ELISA.

RESULTS:

Assessment of technologic quality (methodology) in diagnostic tests demonstrated that sensitivity of CLIA was 0.64 U/ml and the functional sensitivity was <0.9 U/ml. The within-assay and the between-assay imprecisions of different concentrations were all <5%. Recoveries were all in 93-107%. The linear regression equation between expected values and measured values was y=0.0967+1.0093x, correlation coefficient was 0.9996 (p<0.0001). The ROC curve showed that the sensitivity and specificity of the CLIA both were >90%. The area under the curve was 0.992, which was significantly higher than that of ELISA (p<0.05).

CONCLUSION:

The CLIA was the excellent method for EBV-VCA IgM measurement at present and can improve the clinical diagnosis of infectious mononucleosis.

• Authors: Zhenru Feng, Zhiyan Li, Baohuan Sui, Guobing Xu, Tiean Xia
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 354 Issue 1-2 Pg. 77-82 (Apr 2005) ISSN: 0009-8981 [Print] Netherlands
• PMID: 15748602 (Publication Type: Comparative Study, Evaluation Study, Journal Article)

Chemical References

• Antibodies, Viral
• Antigens, Viral
• Immunoglobulin M

Topics

• Adolescent
• Antibodies, Viral (blood)
• Antigens, Viral (immunology)
• Capsid (immunology)
• Child
• Child, Preschool
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Humans
• Immunoenzyme Techniques (methods)
• Immunoglobulin M (blood)
• Infectious Mononucleosis (blood, diagnosis, immunology)
• Linear Models
• Luminescent Measurements (methods)
• Male
• ROC Curve
• Regression Analysis
• Reproducibility of Results
• Sensitivity and Specificity
• Serologic Tests (methods)