Comparison of different inhalational perfluorocarbons in a rabbit model of acute lung injury.

Abstract

The purpose of this study was to investigate the influence of different inhaled perfluorocarbons (PFC) upon pulmonary mechanics and gas exchange in a saline lavage model of acute lung injury. A randomized, controlled animal trial was conducted at the university hospital laboratory. Pulmonary gas exchange (pGE), static compliance (Cst), and basic hemodynamics (heart rate [HR], arterial [AP] and central venous pressures [CVP]) were compared. After induction of lung injury by repeated pulmonary lavage with saline solution, 35 New Zealand rabbits (3 +/- 0.2 kg) were randomized into five groups with seven animals each: 1) conventional ventilated control, 2) perfluorooctane (octane), 3) Perflubron (perfluorooctylbromide [PFOB]), 4) Perfluoro-1,3,5-trimethylcyclohexane (PP 4), and 5) perfluorohexane (hexane). Consecutively, PFC groups were subjected to a 120 minute study period applying mechanical ventilation (tidal volume of 7 ml/kg) in conjunction with PFC performed by a modified halothane vaporizer. Amount of vaporization was controlled by weighing the vaporizer at approximately 25 ml/h/kg body-weight PFC. Controls remained gas ventilated. After injury, PaO2 was control = 53 +/- 13 mbar, octane = 55 +/- 24 mbar, perflubron = 57 +/- 18 mbar, PP4 = 68 +/- 25 mbar, and hexane = 51 +/- 16 mbar. Within the 120 minute period, PaO2 was control = 51 +/- 19 mbar, octane = 42 +/- 6 mbar, perflubron = 40 +/- 11 mbar, PP4 = 47 +/- 10 mbar, and hexane = 60 +/- 8 mbar, respectively. At baseline, after injury, and throughout the study period, pGE and Cst, as well as HR, AP, and CVP, did not significantly differ when compared with octane, PP4, PFOB, and controls (p > 0.05), whereas hexane significantly improved pGE and Cst (p < 0.05). From four different inhaled perfluorocarbons, only perfluorohexane has measureable impact upon gas exchange and lung mechanics when compared with a conventional lung protective ventilation mode.

• Authors: Juergen P Meinhardt, Marc Schmittner, Peter Herrmann, Michael Mailer, Michael Quintel
• Journal: ASAIO journal (American Society for Artificial Internal Organs : 1992) (ASAIO J) 2005 Jan-Feb Vol. 51 Issue 1 Pg. 85-91 ISSN: 1058-2916 [Print] United States
• PMID: 15745141 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Fluorocarbons
• Hydrocarbons, Brominated
• perfluorooctane
• perflexane
• perflubron

Topics

• Administration, Inhalation
• Animals
• Disease Models, Animal
• Female
• Fluorocarbons (administration & dosage, therapeutic use)
• Hemodynamics (drug effects)
• Hydrocarbons, Brominated
• Lung Compliance (drug effects)
• Lung Diseases (therapy)
• Lung Injury
• Pulmonary Gas Exchange (drug effects)
• Rabbits
• Respiration, Artificial
• Tidal Volume (drug effects)