Gradient ion-pair chromatographic method for the determination of iron N,N'-ethylenediamine-di-(2-hydroxy-5-sulfophenylacetate) by high performance liquid chromatography-atmospheric pressure ionization electrospray mass spectrometry.

Abstract

The most effective remedy for iron deficiency is the use of synthetic iron chelates, specifically chelates derived from polyaminecarboxylic acids as EDDHSA (N,N'-ethylenediamine-di-(2-hidroxy-5-sulfophenylacetic) acid). A gradient ion-pair chromatographic method was developed to quantify the total amount of chelated iron in EDDHSA/Fe3+ fertilizers. Two mobile phases were used containing, respectively, 35 and 75% acetonitrile in a 0.005 M tetrabutylammonium hydroxide aqueous solution at pH 6.0. The stationary phase was a reverse phase C-18 column (150mm x 3.9mm i.d., dp = 5 microm). Two chromatographic peaks appeared and were identified by Electrospray Mass Spectrometry. The first peak corresponds to the monomer of EDDHSA/Fe3+ and the second peak has been assigned to condensation molecules. Quality parameters indicate that the method is suitable for the quantification of iron chelate by EDDHSA fertilizers.

• Authors: Sonia García-Marco, Mauro A Cremonini, Pedro Esteban, Felipe Yunta, Lourdes Hernández-Apaolaza, Giuseppe Placucci, Juan J Lucena
• Journal: Journal of chromatography. A (J Chromatogr A) Vol. 1064 Issue 1 Pg. 67-74 (Jan 28 2005) ISSN: 0021-9673 [Print] Netherlands
• PMID: 15729821 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• N,N'-ethylenediamine-di-(2-hidroxy-5-sulfophenylacetic) acid
• Phenylacetates

Topics

• Atmospheric Pressure
• Chromatography, High Pressure Liquid (methods)
• Hydrogen-Ion Concentration
• Phenylacetates (analysis)
• Spectrometry, Mass, Electrospray Ionization (methods)