[Effects of total alkaloids of Tripterygium hypoglaucum Hutch on tk gene of mouse lymphoma cells].

Abstract

OBJECTIVE:

To investigate the effects of total alkaloids of Tripterygium hypoglaucum Hutch (THH) on the tk gene of mouse lymphoma cells.

METHOD:

L5178Y cells were infected with total alkaloids of THH with different concentrations and put into single-cell wells at different time phases. Then the numbers of positive wells were counted and the cell plating efficiency, relative suspension growth and mutation frequency were determined.

RESULT:

Total alkaloids of THH (0.1-2.0 g x L(-1)) induced tk locus mutation with mutation frequency 2-9 times higher than that of spontaneous mutation frequency of L5178Y cells. There were two different phenotypes of mutation colonies, large colony and small colony, but the main colony was large colony. This phenomenon might be related with the mutagenesis of THH.

CONCLUSION:

Total alkaloids of THH can exert toxicity and mutagenic effects on tk gene in L5178Y cells, and there may be range limit in gene mutation.

• Authors: Sheng-xue Liu, Jia Cao, Jian Yuan, Ping Huang, Pei-qiang Shua, Masamitsu Honma
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 28 Issue 10 Pg. 954-7 (Oct 2003) ISSN: 1001-5302 [Print] China
• PMID: 15620187 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Mutagens
• Thymidine Kinase

Topics

• Alkaloids (administration & dosage, isolation & purification, toxicity)
• Animals
• Cell Line, Tumor
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Leukemia L5178 (enzymology, genetics, pathology)
• Mice
• Mutagens (administration & dosage, isolation & purification, toxicity)
• Plants, Medicinal (chemistry)
• Point Mutation
• Thymidine Kinase (genetics)
• Tripterygium (chemistry)