[Effect of HIV Tat protein on CCR5 expression in monocytes and infection with monocyte-tropic HIV strains].

Abstract

OBJECTIVE:

To study the effects of HIV Tat protein on CCR5 expression of monocytes and HIV infection in monocytes.

METHODS:

Membrane expression of CCR5 on monocytes was analyzed by flow cytometry. Stimulated with HIV Tat protein, monocytes were infected with monocyte-tropic HIV(Ba-L) and HIV gag p24 level in the supernatant was measured by ELISA methods.

RESULTS:

HIV Tat protein increased CCR5 expression in human monocytes,which was inhibited by rabbit anti-Tat polyclonal antibody. Tat protein also increased p24 level after monocyte-tropic HIV-1(Ba-L) infected monocytes.

CONCLUSION:

Tat increases CCR5 expression and HIV-1 infection in monocytes, which indicates that HIV Tat might be a key protein in HIV-1 infection.

• Authors: Yi-da Yang, Lin Zheng, Guo-cai Lu, Ya-gang Chen, Maria Salvato
• Journal: Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences (Zhejiang Da Xue Xue Bao Yi Xue Ban) Vol. 33 Issue 6 Pg. 532-4, 545 (11 2004) ISSN: 1008-9292 [Print] China
• PMID: 15586413 (Publication Type: English Abstract, Journal Article)

Chemical References

• Gene Products, tat
• Receptors, CCR5
• tat Gene Products, Human Immunodeficiency Virus

Topics

• Flow Cytometry
• Gene Products, tat (pharmacology)
• HIV
• HIV Infections (metabolism)
• Humans
• Monocytes (metabolism)
• Receptors, CCR5 (biosynthesis, genetics)
• tat Gene Products, Human Immunodeficiency Virus