Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4.

Abstract

The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.

• Authors: Mikako Ito, Tomohiko Takasaki, Ken-Ichiro Yamada, Reiko Nerome, Shigeru Tajima, Ichiro Kurane
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 42 Issue 12 Pg. 5935-7 (Dec 2004) ISSN: 0095-1137 [Print] United States
• PMID: 15583346 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fluorescent Dyes
• RNA, Viral
• Taq Polymerase

Topics

• Dengue (virology)
• Dengue Virus (classification, genetics, isolation & purification)
• Fluorescent Dyes
• Humans
• RNA, Viral (blood)
• Reverse Transcriptase Polymerase Chain Reaction (methods)
• Sensitivity and Specificity
• Taq Polymerase