Abstract | BACKGROUND: METHODS: Rat peritoneal eluate containing 5-6% mast cells were activated by a low concentration of compound 48/80 in a medium containing the vital fluorescent dye, Sulforhodamine-B (SFRM-B, 200 microg/ml), which is engulfed by activated mast cells. The fluorescent image of activated mast cells was captured digitally and the total fluorescent area was analyzed when desloratadine was applied before or after compound 48/80. RESULTS: Mast cells were not activated by desloratadine (10(-4) M), SFRM-B (200 microg/ml), or diluent alone. A low concentration of compound 48/80 (0.125 microg/ml) induced fluorescence, while mast cells lost fluorescent images due to further degranulation on re-exposure to compound 48/80. Desloratadine (10(-8)-10(-4) M), inhibited compound 48/80-induced mast cell degranulation in a concentration-dependent manner. Desloratadine also reduced the loss of fluorescent images due to re-exposure to compound 48/80. CONCLUSIONS:
Desloratadine may have a mast cell stabilizing effect at low concentrations in response to repeated mast cell activation in vitro.
|
Authors | Y H Wang, Y Taché, A G Harris, W Kreutner, A F Daly, J Y Wei |
Journal | Allergy
(Allergy)
Vol. 60
Issue 1
Pg. 117-24
(Jan 2005)
ISSN: 0105-4538 [Print] Denmark |
PMID | 15575942
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Fluorescent Dyes
- Histamine H1 Antagonists, Non-Sedating
- Rhodamines
- lissamine rhodamine B
- p-Methoxy-N-methylphenethylamine
- Loratadine
- desloratadine
|
Topics |
- Animals
- Cell Degranulation
(drug effects)
- Cell Survival
- Dose-Response Relationship, Drug
- Fluorescent Dyes
- Histamine H1 Antagonists, Non-Sedating
(administration & dosage, pharmacology)
- Loratadine
(administration & dosage, analogs & derivatives, pharmacology)
- Male
- Mast Cells
(cytology, drug effects, physiology)
- Peritoneal Cavity
(cytology)
- Rats
- Rats, Sprague-Dawley
- Rhodamines
- p-Methoxy-N-methylphenethylamine
(administration & dosage, antagonists & inhibitors, pharmacology)
|