Abstract | BACKGROUND: Anti-Saccharomyces cerevisiae mannan antibodies have been proposed as a new serological marker associated with Crohn's disease. However, their clinical value is still unclear; furthermore, a standardization of anti-S. cerevisiae mannan measurements is lacking. AIM: In this study, we aimed to assess the correlation between anti-S. cerevisiae mannan detection and specific clinical features in Crohn's disease and ulcerative colitis. Moreover, we tested the concordance of four different anti-S. cerevisiae mannan assays. MATERIALS AND METHODS: RESULTS: With the Lille assay, anti-S. cerevisiae mannan were detected in 100 of 196 patients with Crohn's disease (51%; P < 0.0001 vs. controls), 32 of 197 patients with ulcerative colitis (16%; P < 0.02 vs. controls), and six of 100 controls (6%). No correlation between presence of anti-S. cerevisiae mannan and specific clinical features was found in both ulcerative colitis and Crohn's disease patients. The percentages of anti-S. cerevisiae mannan detected with four different assays ranged from 28 (Bouty) up to 43% (Inova), but these differences did not reach statistical significance. The concordance rate of anti-S. cerevisiae mannan detection in the four assays was very low (11 concordant results of 60 samples, 18.3%) (k = 0.15). No improvement of the concordance rate was obtained by modifying the suggested cut-off values (k = 0.20). CONCLUSION: In this study, we confirm that anti-S. cerevisiae mannan are significantly more frequent in Crohn's disease patients compared with ulcerative colitis patients (P < 0.0001) and controls. However, no correlation with clinical features was found in both ulcerative colitis and Crohn's disease. The low prevalence of anti-S. cerevisiae mannan, at least in our population, and the low concordance rate between different assays, makes the clinical role of this marker questionable.
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Authors | V Annese, A Piepoli, F Perri, G Lombardi, A Latiano, G Napolitano, G Corritore, P Vandewalle, D Poulain, J F Colombel, A Andriulli |
Journal | Alimentary pharmacology & therapeutics
(Aliment Pharmacol Ther)
Vol. 20
Issue 10
Pg. 1143-52
(Nov 15 2004)
ISSN: 0269-2813 [Print] England |
PMID | 15569117
(Publication Type: Clinical Trial, Comparative Study, Journal Article, Randomized Controlled Trial)
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Chemical References |
- Antibodies, Antineutrophil Cytoplasmic
- Antibodies, Fungal
- Mannans
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Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Antibodies, Antineutrophil Cytoplasmic
(analysis)
- Antibodies, Fungal
(analysis)
- Antibody Specificity
- Colitis, Ulcerative
(immunology)
- Crohn Disease
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Male
- Mannans
(immunology)
- Middle Aged
- Saccharomyces cerevisiae
(immunology)
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