Immunoglobulin idiotype expression in reactive lymphoid tissues and B-cell lymphomas.

Abstract

In an investigation of the immunoglobulin idiotypic expression of non-tumour and neoplastic B lymphocytes in situ, fresh-frozen specimens of reactive tonsils, lymph nodes and B-cell malignant lymphomas (B-MLs) from Japanese patients were studied immunohistochemically with 39 different anti-idiotype antibodies. In reactive lymphoid tissues, while idiotype-bearing cells were largely distributed sparsely in follicles and perifollicular areas, some were heavily crowded in particular germinal centres (GCs), suggesting the presence of oligoclonal proliferations of B-cells in GCs. Forty-eight out of 100 B-MLs reacted with anti-idiotype antibodies. This proportion was significantly higher than those reported in Western cases (27-36%), indicating that Japanese B-MLs share public idiotypes much frequently than western cases. The idiotypes demonstrated in these lymphomas, in contrast to those not expressed in any B-ML, were found much commonly in non-tumour lymphocytes, suggesting that such public idiotypes as were common in B-MLs were frequently shared by normal B-lymphocytes.

• Authors: M Shiota, K Kitamura, M Noda, Y Kawanishi, S Hart, R A Miller, S Mori
• Journal: Virchows Archiv. A, Pathological anatomy and histopathology (Virchows Arch A Pathol Anat Histopathol) Vol. 420 Issue 3 Pg. 263-7 ( 1992) ISSN: 0174-7398 [Print] Germany
• PMID: 1553818 (Publication Type: Journal Article)

Chemical References

• Antibodies
• Immunoglobulin Idiotypes

Topics

• Antibodies
• B-Lymphocytes (immunology, pathology)
• Humans
• Hyperplasia
• Immunoenzyme Techniques
• Immunoglobulin Idiotypes (analysis)
• Lymph Nodes (immunology, pathology)
• Lymphoma, T-Cell (immunology, pathology)
• Palatine Tonsil (immunology, pathology)