Isolation of anti-ulcerogenic sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis through bioassay-guided fractionation procedures in rats.

Abstract

The fresh spiny flowers of Centaurea solstitialis ssp. solstitialis (CSS) are used for the treatment of peptic ulcers in Turkey. Ethanol (80%) extract of CSS exhibited significant anti-ulcerogenic effect on the ethanol-induced ulcerogenesis model in rats. The ethanol extract was further fractionated by successive solvent extractions with n-hexane, chloroform, ethyl acetate and n-butanol. All fractions showed significant anti-ulcerogenic activity, however, the effect of the chloroform fraction was found to be more prominent with 99.5% ulcer inhibition. Bioassay-guided fractionation yielded sesquiterpene lactones as the active components. The main components responsible for the activity of the chloroform fraction were determined as chlorojanerin and 13-acetyl solstitialin A which were elucidated by HR-ESI and (1)H, (13)C and 2D NMR spectroscopic techniques.

• Authors: Erdem Yesilada, Ilhan Gürbüz, Erdal Bedir, Irem Tatli, Ikhlas A Khan
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 95 Issue 2-3 Pg. 213-9 (Dec 2004) ISSN: 0378-8741 [Print] Ireland
• PMID: 15507339 (Publication Type: Journal Article)

Chemical References

• Anti-Ulcer Agents
• Lactones
• Plant Extracts
• Sesquiterpenes

Topics

• Animals
• Anti-Ulcer Agents (isolation & purification)
• Biological Assay (methods)
• Centaurea
• Chemical Fractionation (methods)
• Female
• Flowers
• Lactones (isolation & purification, therapeutic use)
• Male
• Plant Extracts (isolation & purification, therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Sesquiterpenes (isolation & purification, therapeutic use)
• Stomach Ulcer (drug therapy)