Comparative biodistribution of potential anti-glioblastoma conjugates [111In]DTPA-hEGF and [111In]Bz-DTPA-hEGF in normal mice.

Abstract

EGF-receptors (EGFR) are overexpressed in gliomas, as well as in tumors of breast, lung, and urinary bladder. For this reason, EGFR may be an attractive target for both visualization and therapy of malignant tumors using radioactive nuclides. Natural ligand of EGFR, epidermal growth factor (EGF) is a small 53-amino-acid protein. Low molecular weight of EGF may enable better intratumoral penetration in comparison to antibodies. [111In]DTPA-EGF was proposed for the targeting of glioblastoma and breast cancer, and its tumor-seeking properties were confirmed in animal studies. The aim of this study was to evaluate how the substitution of heptadentate DTPA for octadentate benzyl-DTPA (Bz-DTPA) effects the biodistribution of indium-labeled human EGF (hEGF) in normal NMRI mice. [111In]DTPA-hEGF and [111In]Bz-DTPA-hEGF, obtained by the coupling of ITC-benzyl-DTPA to hEGF, were injected into the tail vein. At 0.5, 1, 4, and 24 hours postinjection, the animals were sacrificed, and radioactivity in different organs was measured. The blood clearance of both conjugates was fast. The uptake of both conjugates in the liver, spleen, stomach, pancreas, intestines, and submaxillary gland was most likely receptor-mediated. The uptake in a majority of organs was similar. However, indium uptake in the case of [111In]DTPA-hEGF was significantly higher in the kidneys and bones. In conclusion, [111In]Bz-DTPA-hEGF seems to have more favourable in vivo distribution in comparison to [111In]DTPA-hEGF.

• Authors: Vladimir Tolmachev, Anna Orlova, Qichun Wei, Alexander Bruskin, Jörgen Carlsson, Lars Gedda
• Journal: Cancer biotherapy & radiopharmaceuticals (Cancer Biother Radiopharm) Vol. 19 Issue 4 Pg. 491-501 (Aug 2004) ISSN: 1084-9785 [Print] United States
• PMID: 15453964 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright Mary Ann Liebert, Inc.

Chemical References

• 111In-DTPA-human epidermal growth factor
• Indium Radioisotopes
• Isothiocyanates
• Radiopharmaceuticals
• isothiocyanate-benzyl-DTPA
• Epidermal Growth Factor
• Pentetic Acid
• ErbB Receptors

Topics

• Animals
• Epidermal Growth Factor (pharmacokinetics)
• ErbB Receptors (metabolism)
• Female
• Glioblastoma (radiotherapy)
• Indium Radioisotopes (pharmacokinetics)
• Isothiocyanates (pharmacokinetics)
• Mice
• Pentetic Acid (analogs & derivatives, pharmacokinetics)
• Radiopharmaceuticals (pharmacokinetics)