Abstract |
Chitosan microspheres (CMs) were prepared by an ionic gelation process with tripolyphosphate and characterized. Bordetella Bronchiseptica Dermonecrotoxin (BBD), a major virulence factor of a causative agent of atrophic rhinitis (AR), was loaded on to the CMs for nasal vaccination. BBD-loaded CMs were observed as aggregated shapes although unloaded CMs were observed as relatively spherical ones. The average particle size of the BBD-loaded CMs was 4.39 microm. The lower the molecular weight of chitosan and the higher the medium pH, the greater was the release of BBD from the BBD-loaded CMs in vitro due to weaker intermolecular interaction between chitosan and BBD. Tumor necrosis factor alpha and nitric oxide from RAW264.7 cells exposed to BBD-loaded CMs were gradually secreted with time, suggesting that released BBD from CMs had immune stimulating activity of AR vaccine in vitro.
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Authors | Hu-Lin Jiang, In-Kyu Park, Na-Ri Shin, Sang-Gyun Kang, Han-Sang Yoo, Su-Il Kim, Sang-Bong Suh, Toshihiro Akaike, Chong-Su Cho |
Journal | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
(Eur J Pharm Biopharm)
Vol. 58
Issue 3
Pg. 471-6
(Nov 2004)
ISSN: 0939-6411 [Print] Netherlands |
PMID | 15451520
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Bacterial Toxins
- Bacterial Vaccines
- Virulence Factors, Bordetella
- dermonecrotic toxin, Bordetella
- Chitosan
- Transglutaminases
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Topics |
- Adjuvants, Immunologic
(administration & dosage)
- Animals
- Bacterial Toxins
(administration & dosage, immunology)
- Bacterial Vaccines
(administration & dosage, immunology)
- Bordetella bronchiseptica
(immunology)
- Cell Line
- Chitosan
(administration & dosage, immunology)
- Mice
- Microspheres
- Rhinitis, Atrophic
(immunology, prevention & control)
- Swine
- Transglutaminases
(administration & dosage, immunology)
- Virulence Factors, Bordetella
(administration & dosage, immunology)
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