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In vitro study of the immune stimulating activity of an atrophic [correction of athrophic] rhinitis vaccine associated to chitosan microspheres.

Abstract
Chitosan microspheres (CMs) were prepared by an ionic gelation process with tripolyphosphate and characterized. Bordetella Bronchiseptica Dermonecrotoxin (BBD), a major virulence factor of a causative agent of atrophic rhinitis (AR), was loaded on to the CMs for nasal vaccination. BBD-loaded CMs were observed as aggregated shapes although unloaded CMs were observed as relatively spherical ones. The average particle size of the BBD-loaded CMs was 4.39 microm. The lower the molecular weight of chitosan and the higher the medium pH, the greater was the release of BBD from the BBD-loaded CMs in vitro due to weaker intermolecular interaction between chitosan and BBD. Tumor necrosis factor alpha and nitric oxide from RAW264.7 cells exposed to BBD-loaded CMs were gradually secreted with time, suggesting that released BBD from CMs had immune stimulating activity of AR vaccine in vitro.
AuthorsHu-Lin Jiang, In-Kyu Park, Na-Ri Shin, Sang-Gyun Kang, Han-Sang Yoo, Su-Il Kim, Sang-Bong Suh, Toshihiro Akaike, Chong-Su Cho
JournalEuropean journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (Eur J Pharm Biopharm) Vol. 58 Issue 3 Pg. 471-6 (Nov 2004) ISSN: 0939-6411 [Print] Netherlands
PMID15451520 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adjuvants, Immunologic
  • Bacterial Toxins
  • Bacterial Vaccines
  • Virulence Factors, Bordetella
  • dermonecrotic toxin, Bordetella
  • Chitosan
  • Transglutaminases
Topics
  • Adjuvants, Immunologic (administration & dosage)
  • Animals
  • Bacterial Toxins (administration & dosage, immunology)
  • Bacterial Vaccines (administration & dosage, immunology)
  • Bordetella bronchiseptica (immunology)
  • Cell Line
  • Chitosan (administration & dosage, immunology)
  • Mice
  • Microspheres
  • Rhinitis, Atrophic (immunology, prevention & control)
  • Swine
  • Transglutaminases (administration & dosage, immunology)
  • Virulence Factors, Bordetella (administration & dosage, immunology)

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