Abstract |
We studied the ultrastructure of Torulopsis sphaerica yeast cells irradiated with He-Ne laser (lambda = 632.8 nm, dose--460 J/m2) and then cultured for 6 h in the nutrient with 1% glucose by aeration. The length of membranes of endoplasmic reticulum (ER) and the number of its associations with mitochondria (M) and plasma membrane (PM) were measured on ultrathin sections. A distance of less than 50 nm between heterogeneous membranes was considered as an "association". The cells from irradiated cultures are characterized by the following features: 1) the length of cortical ER membranes in relation to cellular perimeter, and the length of perinuclear ER membranes in relation to nuclear perimeter increase, resp., by 21 and 79%; 2) the number of ER-PM associations per cellular section, and that per unit of PM length increase, resp., by 26 and 41%; 3) the number of ER-M association in relation to the total mitochondrial perimeter, and to perimitochondrial ER increase by 80 and 87%, resp. The latter may be associated with Ca2+ uptake by mitochondria associated with ER, which results in activation of respiration and ATP production.
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Authors | V M Manteĭfel', T I Karu |
Journal | Tsitologiia
(Tsitologiia)
Vol. 46
Issue 6
Pg. 498-505
( 2004)
ISSN: 0041-3771 [Print] Russia (Federation) |
Vernacular Title | Uvelichenie kolichestva assotsiatsiĭ éndoplazmaticheskogo retikuluma s mitokhondriiami i plazmaticheskoĭ membranoĭ v drozhzhevykh kletkakh, stimulirovannykh k deleniiu svetom He-Ne-lazera. |
PMID | 15341124
(Publication Type: Comparative Study, English Abstract, Journal Article)
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Chemical References |
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Topics |
- Cell Membrane
(metabolism, radiation effects, ultrastructure)
- Endoplasmic Reticulum
(metabolism, radiation effects, ultrastructure)
- Helium
- Lasers
- Microscopy, Electron
- Mitochondria
(metabolism, radiation effects, ultrastructure)
- Neon
- Yeasts
(metabolism, radiation effects, ultrastructure)
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