Efficient priming of simian/human immunodeficiency virus (SHIV)-specific T-cell responses with DNA encoding hybrid SHIV/hepatitis B surface antigen particles.

Abstract	Recent efforts to design an human immunodeficiency virus type 1 (HIV-1) vaccine candidate have focused on means of eliciting anti-viral T-cell responses. We tried to improve the immunogenicity of DNA vaccines by designing hybrid DNA constructs encoding hepatitis B surface antigen (HBsAg) fused to antigenic domains of simian/human immunodeficiency virus (SHIV 89.6P). Immunisation with hybrid DNA induced both effector and long-lasting precursor T-cells. Following boosting with a recombinant modified vaccinia Ankara (rMVA) producing full-length SIV and HIV antigens, it appeared that priming with hybrid DNA had increased virus-specific T-cell responses in terms of both the number of virus-specific IFN-gamma-secreting T-cells and virus-specific lymphoproliferation. After intrarectal challenge with SHIV 89.6P, immunised animals demonstrated early control of SHIV 89.6P replication and stable CD4+ T-cell counts.
Authors	Anne-Laure Puaux, Delphine Marsac, Stéphane Prost, Mandal K Singh, Patricia Earl, Bernard Moss, Roger Le Grand, Yves Riviere, Marie-Louise Michel
Journal	Vaccine (Vaccine) Vol. 22 Issue 27-28 Pg. 3535-45 (Sep 09 2004) ISSN: 0264-410X [Print] Netherlands
PMID	15315833 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	AIDS Vaccines DNA Probes Hepatitis B Surface Antigens SAIDS Vaccines Vaccines, DNA Vaccines, Subunit Interferon-gamma DNA
Topics	AIDS Vaccines (therapeutic use) Animals Area Under Curve CD4-Positive T-Lymphocytes (immunology) Cell Division (drug effects) Cytomegalovirus (genetics) DNA (genetics, immunology) DNA Probes Enzyme-Linked Immunosorbent Assay Genetic Vectors HIV (immunology) Hepatitis B Surface Antigens (genetics, immunology) Immunization, Secondary Interferon-gamma Kinetics Macaca mulatta Reverse Transcriptase Polymerase Chain Reaction SAIDS Vaccines (therapeutic use) Simian Immunodeficiency Virus (immunology) T-Lymphocytes (drug effects, immunology) Vaccines, DNA (therapeutic use) Vaccines, Subunit (therapeutic use) Vaccinia (immunology) Virus Replication

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