Distribution of antioxidant enzymes in developing human lung, respiratory distress syndrome, and bronchopulmonary dysplasia.

Abstract

We studied cell-specific protein expression of all the major antioxidant enzymes (AOEs) and related proteins, such as copper-zinc superoxide dismutase (CuZnSOD), manganese SOD (MnSOD), extracellular SOD (ECSOD), catalase, the heavy and light chains of gamma-glutamylcysteine synthetase (gamma-GCS-l and gamma-GCS-h, also called glutamate cysteine ligase), the rate-limiting enzyme in glutathione synthesis, hemeoxygenase-1 (HO-1), and thioredoxin (Trx), in developing human lung, respiratory distress syndrome, and bronchopulmonary dysplasia by immunohistochemistry. Generally, after 17 weeks of gestational age, MnSOD was predominantly expressed in bronchial epithelium, alveolar epithelium, and macrophages, CuZnSOD was expressed in bronchial epithelium, ECSOD was expressed in bronchial epithelium, vascular endothelium, and the extracellular matrix, catalase was expressed in bronchial epithelium and alveolar macrophages, gamma-GCS-h was expressed in bronchial epithelium and endothelium, and gamma-GCS-l was expressed in bronchial epithelium. Trx was restricted to bronchial epithelium and to a lesser extent to alveolar macrophages, and HO-1 found in alveolar macrophages. Basically, the expression of these enzymes was similar in normal and diseased lung. It can be concluded that various AOEs and related proteins differ in their distribution and expression in lung before term, but generally it seems that infants are better adapted to high oxygen tension than might be expected.

• Authors: Riitta Kaarteenaho-Wiik, Vuokko L Kinnula
• Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (J Histochem Cytochem) Vol. 52 Issue 9 Pg. 1231-40 (Sep 2004) ISSN: 0022-1554 [Print] United States
• PMID: 15314090 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright The Histochemical Society, Inc.

Chemical References

• Antioxidants
• Membrane Proteins
• Thioredoxins
• Catalase
• HMOX1 protein, human
• Heme Oxygenase (Decyclizing)
• Heme Oxygenase-1
• Superoxide Dismutase
• Glutamate-Cysteine Ligase

Topics

• Antioxidants (metabolism)
• Bronchopulmonary Dysplasia (embryology, enzymology, pathology)
• Catalase (metabolism)
• Female
• Gene Expression Regulation, Developmental
• Gene Expression Regulation, Enzymologic
• Glutamate-Cysteine Ligase (metabolism)
• Heme Oxygenase (Decyclizing) (metabolism)
• Heme Oxygenase-1
• Humans
• Infant
• Infant, Newborn
• Lung (embryology, enzymology, pathology)
• Male
• Membrane Proteins
• Pregnancy
• Pregnancy Outcome
• Respiratory Distress Syndrome, Newborn (embryology, enzymology, pathology)
• Superoxide Dismutase (metabolism)
• Thioredoxins (metabolism)