Cloning of murine IL-22 receptor alpha 2 and comparison with its human counterpart.

Abstract

We have identified the mouse and rat homologs of human interleukin-22 receptor alpha 2 (IL-22R alpha 2) and compared the localization, structure, and expression of the encoding murine and human genes. The mouse IL-22R alpha 2-encoding gene is located on chromosome 10A3 between, like in human, the genes for interferon-gamma R1 and IL-20R1. It spans a region of approximately 10 kb therefore being three times shorter than the human gene. Although the overall gene structure in both species is similar, the mouse gene lacks a counterpart to the third coding exon of the human gene known to be alternatively spliced. Like in human, mouse and rat IL-22R alpha 2 exist only as soluble receptors as deduced from the lack of transmembrane and intracellular domains encoding sequences. Quantitative expression analyses showed, analogically to the human system, a limited tissue distribution of mouse IL-22R alpha 2 mRNA. Differential modulation of IL-22R alpha 2 mRNA expression was observed upon systemic inflammation in mice in spleen, thymus, and lymph node.

• Authors: B Weiss, K Wolk, B H Grünberg, H-D Volk, W Sterry, K Asadullah, R Sabat
• Journal: Genes and immunity (Genes Immun) Vol. 5 Issue 5 Pg. 330-6 (Aug 2004) ISSN: 1466-4879 [Print] England
• PMID: 15201862 (Publication Type: Comparative Study, Journal Article)

Chemical References

• IL22RA2 protein, human
• RNA, Messenger
• Receptors, Cell Surface
• Receptors, Interleukin
• interleukin-22 receptor

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Chromosome Mapping
• Chromosomes, Human, Pair 6 (genetics)
• Cloning, Molecular
• Gene Expression
• Humans
• Mice
• Mice, Inbred BALB C
• Molecular Sequence Data
• RNA, Messenger (analysis, metabolism)
• Rats
• Receptors, Cell Surface (genetics)
• Receptors, Interleukin (genetics, metabolism)
• Sequence Homology, Amino Acid