Abstract |
Daphne genkwa (Thymelaeaceae) has been used as a folk medicine in China. We investigated the effects of D. genkwa and Jyu-So-To on various pharmacologic models in mice including the azoxymethane (AOM)-induced colonic aberrant crypt focus formation assay, ornithine decarboxylase (ODC) activity assay, and two types of mouse ear swelling model. Administration of 236.3 ppm of Jyu-So-To in drinking water significantly suppressed AOM-induced colonic aberrant crypt focus formation (p < 0.05), with an inhibitory ratio of 46.7%. The effects of several extracts with organic solvents of D. genkwa on murine epidermal ODC activity were examined. In particular, the inhibitory ratio of the n-hexane extract was 30.8%. In the 12-O-Tetradecanoylphorbol-13-acetate (TPA)-induced ear edema model in mice, the methanol extract resulted in 56.3% inhibition compared with the control. On the other hand, there are two peaks of responses at 1 h (immediate-phase reaction; IPR) and 24 h (late-phase reaction) in biphasic cutaneous reactions, which are enhanced in the dinitrofluorobenzene model ( DNFB). The water extract of D. genkwa clearly inhibited the IPR ear swelling. These results suggest that D. genkwa and Jyu-So-To should be a promising source of antitumor, antiinflammatory, and antiallergy agents.
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Authors | Hisahiro Kai, Takehiko Koine, Masaki Baba, Toru Okuyama |
Journal | Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
(Yakugaku Zasshi)
Vol. 124
Issue 6
Pg. 349-54
(Jun 2004)
ISSN: 0031-6903 [Print] Japan |
PMID | 15170070
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Anti-Allergic Agents
- Anti-Inflammatory Agents
- Antineoplastic Agents, Phytogenic
- Drugs, Chinese Herbal
- Plant Extracts
- Dinitrofluorobenzene
- Ornithine Decarboxylase
- Azoxymethane
- Tetradecanoylphorbol Acetate
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Topics |
- Animals
- Anti-Allergic Agents
- Anti-Inflammatory Agents
- Antineoplastic Agents, Phytogenic
- Azoxymethane
- Colonic Neoplasms
(chemically induced, prevention & control)
- Daphne
- Dinitrofluorobenzene
- Drugs, Chinese Herbal
(pharmacology, therapeutic use)
- Ear Diseases
(chemically induced, prevention & control)
- Edema
(chemically induced, prevention & control)
- Female
- Hypersensitivity
(etiology, prevention & control)
- Male
- Mice
- Mice, Inbred Strains
- Ornithine Decarboxylase
(metabolism)
- Phytotherapy
- Plant Extracts
(pharmacology, therapeutic use)
- Tetradecanoylphorbol Acetate
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