[Role of p38 mitogen-activated protein kinase signal transduction pathway in the acute lung injury of severely burned rats].

Abstract

OBJECTIVE:

To investigate the role of p38 mitogen-activated protein kinase (MAPK) signal transduction pathway in the acute lung injury of severely burned rats.

METHODS:

Forty-eight adult healthy rats were randomly divided into three groups: sham group, burn control group, and burn + SB203580 group. A third-degree burns over 30% total body surface area rat model was used and pulmonary capillary permeability, lung water content, pulmonary histology and p38 MAPK activity were measured at 24 hours postburn.

RESULTS:

Burn trauma resulted in increased pulmonary capillary leakage permeability (42.5 +/- 4.7 vs. 12.1 +/- 1.4, P < 0.01), elevated lung water content (P < 0.05), and worsen histologic condition. There was a significant activation of p38 MAPK at 24 hours postburn compared with control. SB203580 inhibited the activation of p38 MAPK, reduced the pulmonary capillary leakage permeability (24.7 +/- 2.9 vs. 42.5 +/- 4.7, P < 0.01), decreased lung water content, and prevented burn-mediated lung injury.

CONCLUSION:

The activation of p38 MAPK is one important aspect of the signaling event that contributes to burn-induced lung injury.

• Authors: Xu-lin Chen, Zhao-fan Xia, Duo Wei, Dao-feng Ben, Guang-qing Wang, Sheng Han
• Journal: Zhonghua wai ke za zhi [Chinese journal of surgery] (Zhonghua Wai Ke Za Zhi) Vol. 42 Issue 7 Pg. 388-90 (Apr 07 2004) ISSN: 0529-5815 [Print] China
• PMID: 15144663 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Inhibitors
• Imidazoles
• Pyridines
• Mitogen-Activated Protein Kinases
• p38 Mitogen-Activated Protein Kinases
• SB 203580

Topics

• Animals
• Blotting, Western
• Burns (enzymology, physiopathology)
• Enzyme Activation (drug effects)
• Enzyme Inhibitors (pharmacology)
• Imidazoles (pharmacology)
• Lung (pathology, physiopathology)
• Male
• Mitogen-Activated Protein Kinases (antagonists & inhibitors, metabolism, physiology)
• Pyridines (pharmacology)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Respiratory Distress Syndrome (enzymology, physiopathology)
• Signal Transduction (drug effects, physiology)
• p38 Mitogen-Activated Protein Kinases