Abstract | BACKGROUND AND AIM: METHODS:
Gastric acid reflux esophagitis was induced in Wistar rats by ligating the transitional region between the forestomach and the glandular portion, and by covering the duodenum near the pyloric ring with a small piece of an 18Fr Nélaton catheter. Epithelial cell proliferation was assessed by bromodeoxyuridine ( BrdU) uptake. Expression of TGF-alpha and EGF-R mRNA and protein was assessed by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. RESULTS: Esophageal lesions were observed in the lower and middle esophagus. Histologically, a significant increase in mucosal thickening with elongation of lamina propria papillae and basal cell hyperplasia was observed. The BrdU labeling index was significantly increased from 2.7 +/- 1.0 in normal mucosa and 2.8 +/- 1.2 in background mucosa adjacent to the esophageal lesion, to 60.3 +/- 32.7 in the lesions of chronic esophagitis. Expression of TGF-alpha and EGF-R mRNA in the esophageal lesion significantly increased compared to those in the control and background tissue, whereas treatment with rabeprazole significantly inhibited increases in TGF-alpha and EGF-R mRNA expression. According to immunohistochemical study, TGF-alpha and EGF-R revealed strong expression in esophageal lesions compared with control and background mucosa. The superficial layer of the esophagus was strongly positive for TGF-alpha and most cells in regions of basal hyperplasia had a positive reaction for EGF-R in the esophagitis lesion. CONCLUSION:
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Authors | Yasuhiro Fujiwara, Kazuhide Higuchi, Masaki Hamaguchi, Takashi Takashima, Toshio Watanabe, Kazunari Tominaga, Nobuhide Oshitani, Takayuki Matsumoto, Tetsuo Arakawa |
Journal | Journal of gastroenterology and hepatology
(J Gastroenterol Hepatol)
Vol. 19
Issue 5
Pg. 521-7
(May 2004)
ISSN: 0815-9319 [Print] Australia |
PMID | 15086595
(Publication Type: Journal Article)
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Chemical References |
- RNA, Messenger
- Transforming Growth Factor alpha
- Epidermal Growth Factor
- ErbB Receptors
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Topics |
- Amino Acid Sequence
- Analysis of Variance
- Animals
- Blotting, Western
- Chronic Disease
- Disease Models, Animal
- Epidermal Growth Factor
(biosynthesis)
- Epithelial Cells
(metabolism, pathology)
- ErbB Receptors
(biosynthesis)
- Esophagitis, Peptic
(metabolism, pathology)
- Esophagus
(metabolism, pathology)
- Gastrointestinal Hemorrhage
(etiology, pathology)
- Gene Expression
- Immunoenzyme Techniques
- Ligation
- Male
- Molecular Sequence Data
- RNA, Messenger
(analysis)
- Rats
- Rats, Wistar
- Reverse Transcriptase Polymerase Chain Reaction
- Transforming Growth Factor alpha
(biosynthesis)
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