Two RNA polymerase complexes from vesicular stomatitis virus-infected cells that carry out transcription and replication of genome RNA.

Abstract

By immunoaffinity column chromatography, we have purified two RNA polymerase complexes, the transcriptase and replicase, from vesicular stomatitis virus-infected baby hamster kidney cells. The transcriptase is a multiprotein complex, containing the virus-encoded RNA polymerase L and P proteins, and two cellular proteins, translation elongation factor-1alpha and heat-shock protein 60. In addition, the complex contains a submolar amount of cellular mRNA cap guanylyltransferase. The replicase, on the other hand, is a complex containing the viral proteins, L, P, and the nucleocapsid (N), but lacking elongation factor-1alpha, heat-shock protein 60, and guanylyltransferase. The transcriptase complex synthesizes capped mRNAs and initiates transcription at the first gene (N) start site, whereas the replicase complex initiates RNA synthesis at the precise 3' end of the genome RNA and synthesizes encapsidated replication products in the presence of the N-P complex. We propose that two RNA polymerase complexes that differ in their content of virally and host-encoded proteins are separately responsible for transcription and replication of vesicular stomatitis virus genome RNA.

• Authors: Kaustubha R Qanungo, Daniel Shaji, Manjula Mathur, Amiya K Banerjee
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 101 Issue 16 Pg. 5952-7 (Apr 20 2004) ISSN: 0027-8424 [Print] United States
• PMID: 15069200 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• RNA, Viral
• DNA-Directed RNA Polymerases

Topics

• Animals
• Cell Line
• Chromatography, Affinity
• Cricetinae
• DNA-Directed RNA Polymerases (isolation & purification, physiology)
• Genome, Viral
• RNA, Viral (biosynthesis)
• Transcription, Genetic (physiology)
• Vesicular stomatitis Indiana virus (enzymology)