Abstract |
Cells of transplantable MC38 colon carcinoma of C57BL/6 mice were adapted to growth in vitro as the MC38/0 cell line. Along the establishing process, MC38/0 cells preserved their tumorigenicity. After transduction with a retroviral vector carrying murine interleukin 12 (mIL-12) genes and further selection, stable MC38/ IL-12 transductant cells were obtained. These cells produced IL-12 (approx. 2500 ng/ml/5x10(5) cells/48 h) as evaluated in the optimized bioassay. After subcutaneous inoculation into syngeneic mice, the IL-12-modified cells demonstrated reduced tumorigenicity as compared to parental MC38/0 cells. Mice that rejected the MC38/ IL-12 tumour became protected against subsequent challenge with MC38/0 cells. The obtained data indicate that the IL-12-transduced murine colon carcinoma cells could be used both as a model tumour for the study of mechanisms of anticancer immunity and/or as an adjuvant to cancer vaccines.
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Authors | E Pajtasz-Piasecka, A Szyda, J Rossowska, A Krawczenko, M Indrová, P Grabarczyk, P Wysocki, A Mackiewicz, D Duś |
Journal | Folia biologica
(Folia Biol (Praha))
Vol. 50
Issue 1
Pg. 7-14
( 2004)
ISSN: 0015-5500 [Print] Czech Republic |
PMID | 15055737
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Luminescent Proteins
- Green Fluorescent Proteins
- Interleukin-12
- Interferon-gamma
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Topics |
- Animals
- Cell Line, Tumor
- Colonic Neoplasms
(genetics, immunology, metabolism, pathology)
- Cytokines
(metabolism)
- Female
- Genes, MHC Class I
- Genetic Vectors
- Green Fluorescent Proteins
- Interferon-gamma
(metabolism)
- Interleukin-12
(genetics, metabolism)
- Luminescent Proteins
(genetics, metabolism)
- Mice
- Mice, Inbred C57BL
- Retroviridae
(genetics)
- Transduction, Genetic
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