A poliomyelitis model through mucosal infection in transgenic mice bearing human poliovirus receptor, TgPVR21.

Abstract

Transgenic mice bearing the human poliovirus receptor (TgPVR) are less susceptible to oral inoculation, although they are susceptible to parenteral inoculation. We investigated the susceptibility of TgPVR 21 line [Arch. Virol. 130 (1994) 351] to poliovirus through various mucosal routes. Intranasal inoculation of a neurovirulent Mahoney strain (OM1) caused flaccid paralysis with viral replication in the central nervous system at a dose of 10(6) cell culture infectious dose (CCID50), in contrast, no paralysis following oral or intragastric inoculation of the same dose. Intranasal inoculation of a vaccine strain, Sabin 1, at 10(6) CCID50, resulted in no paralysis. Initial replication of poliovirus in the nasal cavity was confirmed by virus isolation and detection of negative-stranded replicative intermediates by RT-PCR and viral antigens using a high-sensitive immunohistochemistry and genome/transcripts by in situ hybridization. Poliovirus-specific IgG antibodies were elevated in the sera of surviving TgPVR21. This model can be used as a mucosal infection model and for differentiation of neurovirulent and attenuated poliovirus strains.

• Authors: Noriyo Nagata, Takuya Iwasaki, Yasushi Ami, Yuko Sato, Ikuyoshi Hatano, Ayako Harashima, Yuriko Suzaki, Takao Yoshii, Tsutomu Hashikawa, Tetsutaro Sata, Yoshinobu Horiuchi, Satoshi Koike, Takeshi Kurata, Akio Nomoto
• Journal: Virology (Virology) Vol. 321 Issue 1 Pg. 87-100 (Mar 30 2004) ISSN: 0042-6822 [Print] United States
• PMID: 15033568 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Antigens, Viral
• Membrane Proteins
• Poliovirus Vaccine, Oral
• Receptors, Virus
• poliovirus receptor

Topics

• Administration, Intranasal
• Animals
• Antibodies, Viral (blood)
• Antigens, Viral (analysis)
• Brain (pathology, virology)
• Central Nervous System (pathology, virology)
• Disease Models, Animal
• Disease Susceptibility
• Female
• Immunohistochemistry
• Male
• Membrane Proteins
• Mice
• Mice, Transgenic
• Nasal Mucosa (pathology, virology)
• Paralysis (pathology, virology)
• Poliomyelitis (blood, pathology, virology)
• Poliovirus (pathogenicity, physiology)
• Poliovirus Vaccine, Oral (administration & dosage)
• Receptors, Virus (genetics)
• Spinal Cord (pathology, virology)
• Time Factors