Forskolin promotes astroglial differentiation of human central neurocytoma cells.

Abstract

Human central neurocytoma is a kind of the brain tumors that are usually found in anterior part of the lateral ventricles. In this study, we established conditions that allowed proliferation of neurocytoma cells culture and analyzed characteristics of neurocytoma cells in vitro. For in vitro, a condition that used for culturing neural stem cells and contained basic fibroblast growth factor (bFGF) provided high proliferation. RT-PCR analaysis showed that nestin was found in neurocytoma cells, indicating that the neurocytomas possess neural stem cell properties. Interestingly, treatment of neurocytoma cells with forskolin increased expression of glial fibrillary acidic protein with a concomitant decrease in the nestin expression. Forskolin also induced morphological changes of neurocytoma cells to adopt an astrocyte-like phenotype. The results suggest that neurocyotma cells may have properties of multipotent neural stem cells.

• Authors: Bum Jun Kim, Sung Soo Kim, Young Im Kim, Sun Ha Paek, Young Don Lee, Haeyoung Suh-Kim
• Journal: Experimental & molecular medicine (Exp Mol Med) Vol. 36 Issue 1 Pg. 52-6 (Feb 29 2004) ISSN: 1226-3613 [Print] United States
• PMID: 15031671 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Intermediate Filament Proteins
• NES protein, human
• Nerve Tissue Proteins
• Nestin
• Fibroblast Growth Factor 2
• Colforsin

Topics

• Animals
• Astrocytes (cytology, physiology)
• Cell Differentiation (drug effects)
• Cell Proliferation
• Cell Shape
• Colforsin (pharmacology, therapeutic use)
• Fibroblast Growth Factor 2 (pharmacology)
• Humans
• Intermediate Filament Proteins (metabolism)
• Nerve Tissue Proteins (metabolism)
• Nestin
• Neurocytoma (drug therapy, pathology)
• Tumor Cells, Cultured