Abstract |
Antisense oligonucleotides can selectively block disease-causing genes due to the specificity of the Watson-Crick base-pairing mechanism of action. A genome-wide view of antisense technology is illustrated via protein kinase A RI alpha antisense. Complementary DNA microarray analysis of the RI alpha antisense-induced expression profile shows the up- and down-regulation of clusters of coordinately expressed genes that define the molecular portrait of a reverted tumor cell phenotype. This global view broadens the horizons of antisense technology; it advances the promise of antisense beyond a single target gene to the whole cell and the whole organism. Along with recent rapid advances in oligonucleotide technologies-including new chemical and biological understanding of more sophisticated nucleic acid drugs- oligonucleotide-based gene silencing offers not only an exquisitely specific genetic tool for exploring basic science but also an exciting possibility for treating and preventing cancer and other diseases.
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Authors | Yoon S Cho-Chung |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 1697
Issue 1-2
Pg. 71-9
(Mar 11 2004)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 15023351
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S., Review)
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Chemical References |
- Antineoplastic Agents
- Cyclic AMP-Dependent Protein Kinase RIalpha Subunit
- DNA, Complementary
- Oligonucleotides, Antisense
- PRKAR1A protein, human
- Cyclic AMP-Dependent Protein Kinases
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Clinical Trials as Topic
- Cyclic AMP-Dependent Protein Kinase RIalpha Subunit
- Cyclic AMP-Dependent Protein Kinases
(antagonists & inhibitors, genetics)
- DNA, Complementary
(analysis)
- Down-Regulation
- Gene Expression Regulation, Neoplastic
- Humans
- Neoplasms
(drug therapy, enzymology, genetics, pathology)
- Oligonucleotide Array Sequence Analysis
- Oligonucleotides, Antisense
(chemistry, genetics, pharmacology)
- Phenotype
- Up-Regulation
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