Abstract | BACKGROUND: OBJECTIVES: METHODS: Variable domain of heavy chain-epsilon constant domain 1 cDNAs were amplified from peripheral blood using a two-step semi-nested PCR, cloned and sequenced. RESULTS: The VH gene family usage in subject A was broadly based, but there were two clusters of sequences using genes VH 3-9 and 3-11 with unusually low levels of somatic mutations, 0-3%. Subject B repeatedly used VH 1-69 and subject C repeatedly used VH 1-02, 1-46 and 5a genes. Most clones were highly mutated being only 86-95% homologous to their germline VH gene counterparts and somatic mutations were more abundant at the complementarity determining rather than framework regions. Multiple sequence alignment revealed both repeated use of particular VH genes as well as clonal relatedness among clusters of IgE transcripts. CONCLUSION: In contrast to previous studies we observed no preferred VH gene common to IgE transcripts of the three subjects allergic to grass pollen. Moreover, most of the VH gene characteristics of the IgE transcripts were consistent with oligoclonal antigen-driven IgE responses.
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Authors | J M Davies, R E O'Hehir |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 34
Issue 3
Pg. 429-36
(Mar 2004)
ISSN: 0954-7894 [Print] England |
PMID | 15005737
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Allergens
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Receptor-CD3 Complex, Antigen, T-Cell
- Immunoglobulin E
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Topics |
- Adult
- Allergens
(immunology)
- Antigen Presentation
- B-Lymphocytes
(immunology)
- Base Sequence
- Female
- Genes, Immunoglobulin
- Humans
- Immunoglobulin E
(genetics, immunology)
- Immunoglobulin Heavy Chains
(genetics)
- Immunoglobulin Variable Region
(genetics)
- Male
- Middle Aged
- Molecular Sequence Data
- Mutation
- Poaceae
- Pollen
- Receptor-CD3 Complex, Antigen, T-Cell
(genetics)
- Rhinitis, Allergic, Seasonal
(genetics, immunology)
- Sequence Alignment
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