Chondroitin sulfate of appican, the proteoglycan form of amyloid precursor protein, produced by C6 glioma cells interacts with heparin-binding neuroregulatory factors.

Abstract

Appican produced by rat C6 glioma cells, the chondroitin sulfate (CS) proteoglycan form of the amyloid precursor protein, contains an E disaccharide, -GlcUA-GalNAc(4,6-O-disulfate)-, in its CS chain. In this study, the appican CS chain from rat C6 glioma cells was shown to specifically bind several growth/differentiation factors including midkine (MK) and pleiotrophin (PTN). In contrast, the appican CS from SH-SY5Y neuroblastoma cells contained no E disaccharide and showed no binding to either MK or PTN. These findings indicate that the E motif is essential in the interaction of the appican CS chain with growth/differentiation factors, and suggest that glial appican may mediate the regulation of neuronal cell adhesion and migration and/or neurite outgrowth.

• Authors: Yuko Umehara, Shuhei Yamada, Shuji Nishimura, Junichi Shioi, Nikolaos K Robakis, Kazuyuki Sugahara
• Journal: FEBS letters (FEBS Lett) Vol. 557 Issue 1-3 Pg. 233-8 (Jan 16 2004) ISSN: 0014-5793 [Print] England
• PMID: 14741373 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Amyloid beta-Protein Precursor
• Carrier Proteins
• Cytokines
• Glycosaminoglycans
• Neoplasm Proteins
• Nerve Tissue Proteins
• Oligosaccharides
• Proteoglycans
• appican
• pleiotrophin
• Midkine
• Heparin

Topics

• Amyloid beta-Protein Precursor
• Carbohydrate Sequence
• Carrier Proteins (metabolism)
• Cytokines (metabolism)
• Glioma
• Glycosaminoglycans (chemistry, metabolism)
• Heparin (metabolism)
• Humans
• Midkine
• Molecular Sequence Data
• Neoplasm Proteins (metabolism)
• Nerve Tissue Proteins (metabolism)
• Neuroblastoma
• Oligosaccharides (chemistry)
• Proteoglycans (biosynthesis, chemistry, metabolism)
• Tumor Cells, Cultured