[The expression and clinical implication of inducible nitric oxide synthase mRNA in laryngeal carcinoma and hypopharyngeal carcinoma].

Abstract

OBJECTIVE:

To study the expression of inducible nitric oxide synthase(iNOS) mRNA in laryngeal carcinoma and hypopharyngeal carcinoma and its clinical significance.

METHOD:

The tissue samples (laryngeal carcinoma, 40; hypopharyngeal carcinoma, 12; adjacent tissue, 50; laryngeal papilloma, 10; amyloidosis of larynx, 2; and normal laryngeal mucosa, 7) were examined by reverse transcription-polymerase chain reaction(RT-PCR).

RESULT:

1. The target gene of iNOS were detected in 41 of 52 tumor tissues, 10 of 50 adjacent tissues, 5 of 12 benign tumor tissues and 0 of 7 normal tissues. The difference was significant (P < 0.0001, P < 0.005, P < 0.0001); 2. No significant difference was found among the expression of iNOSmRNA in different primary locations, there was significant statistical difference between the early clinical stages (T1-T2) and the advanced stages (T3-T4); 3. The poorly differentiated tumor cell showed a higher iNOSmRNA expression than that in moderate differentiated tumor cell (P < 0.05) and the iNOSmRNA expression in the lymph node metastasis group was higher than the non-metastasis group (P < 0.05).

CONCLUSION:

It suggests that iNOS gene expression occurs in human laryngeal carcinoma and hypopharyngeal carcinoma, it might involve in the development of head and neck neoplasms in gene level by generating NO.

• Authors: Xiaodong Du, Xinyong Luan, Xinliang Pang, Chang Shu, Feng Wang, Jindong Ren, Chaohui Huang
• Journal: Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology (Lin Chuang Er Bi Yan Hou Ke Za Zhi) Vol. 17 Issue 10 Pg. 612-4 (Oct 2003) China
• PMID: 14727435 (Publication Type: English Abstract, Journal Article)

Chemical References

• RNA, Messenger
• NOS2 protein, human
• Nitric Oxide Synthase
• Nitric Oxide Synthase Type II

Topics

• Aged
• Female
• Humans
• Hypopharyngeal Neoplasms (genetics, metabolism)
• Laryngeal Neoplasms (genetics, metabolism)
• Male
• Middle Aged
• Nitric Oxide Synthase (biosynthesis, genetics)
• Nitric Oxide Synthase Type II
• Papilloma (genetics, metabolism)
• RNA, Messenger (biosynthesis, genetics)
• Reverse Transcriptase Polymerase Chain Reaction