Applications of T-lymphoma labeled with fluorescent quantum dots to cell tracing markers in mouse body.

Abstract

Photoluminescent semiconductor quantum dots (QDs) are novel nanometer-size probes that have found bioimaging. Here we imaged a cell line of mouse lymphocytes. QDs were actively taken into the target cells by endocytotic pathways. The fluorescence of QDs held in the endosomes could be studied for more than a week and remained stable luminescence against cell activation induced by concanavalin A, phytohemagglutinin, phorbol myristate acetate, and calcium ionophore A23187. These results suggested that QD-labeling was stable and did not affect either cell activation or cell function. When QD-labeled cells were intravenously injected into mouse, they remained in the peripheral blood in a concentration of approximately 10% up to 5 days after injection using both fluorescence microscopy and flow cytometry. In addition, approximately 20% of QDs were detected in the kidneys, liver, lung, and spleen and could still be observed 7 days after injection. These results suggested that fluorescent probes of QDs might be useful as bioimaging tools for tracing target cells over the period of a week in vivo.

• Authors: Akiyoshi Hoshino, Ken-ichi Hanaki, Kazuo Suzuki, Kenji Yamamoto
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 314 Issue 1 Pg. 46-53 (Jan 30 2004) ISSN: 0006-291X [Print] United States
• PMID: 14715244 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor

Topics

• Animals
• BALB 3T3 Cells
• Biomarkers, Tumor (blood, metabolism)
• Cell Line, Tumor
• Cell Transplantation (methods)
• Endosomes (metabolism, pathology)
• Lymphoma, T-Cell (metabolism, pathology)
• Male
• Mice
• Microscopy, Fluorescence (instrumentation, methods)
• Organ Specificity
• Quantum Dots
• Staining and Labeling (methods)
• Tissue Distribution