[The activity of blood cholinesterase in rats exposed to dimethypo after drug intervention].

Abstract

OBJECTIVE:

To investigate the activity of ChE in rats poisoned by dimehypo and then treated with pralidoxime methylchloride or unithiol.

METHOD:

Rats were divided into control group (dimehypo); intervention groups [dimehypo plus pralidoxime methylchloride or dimehypo plus unithiol (sodium dimercaptopropanesulphonate)]. Rats were dosed with 4 different doses of dimehypo: 1/16, 1/8, 1/4 and 1/2 of LD50 respectively(the LD50 of dimehypo is 342 mg/kg). After being poisoned with dimehypo orally, rats were immediately injected intramuscularly with pralidoxime methylchloride or unithiol. The activity of ChE in blood was detected before and 1/2, 1, 2, 4 and 24 h after poisoning in dimehypo and intervention groups.

RESULT:

The ChE activity of four dose subgroups at 1 h after poisoning were (1.04 +/- 0.21), (0.84 +/- 0.12), (0.71 +/- 0.12), (0.66 +/- 0.07) U/ml respectively; the ChE activity of pralidoxime methylchloride intervention groups were (1.01 +/- 0.18), (1.17 +/- 0.11), (1.01 +/- 0.04), (1.03 +/- 0.12) U/ml respectively; and the ChE activity of unithiol intervention groups were (1.15 +/- 0.15), (1.26 +/- 0.27), (1.08 +/- 0.08), (1.04 +/- 0.12) U/ml respectively. The inhibited ChE in blood was recovered by either treatment with pyraldoxime methylchloride or unithiol. These two drugs had similar effects of recovering the activity of ChE(P > 0.05), but at higher doses(1/4 and 1/2 of LD50) the effects of both were not so good.

CONCLUSION:

Pralidoxime methylchloride and unithiol could partly recover the activity of ChE inhibited by dimehypo.

• Authors: Weiguo Wan, Mailing Xu, Hejian Zou, Ailing Lu, Xinyu Shen, Yuming Chen
• Journal: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases (Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi) Vol. 20 Issue 6 Pg. 419-21 (Dec 2002) ISSN: 1001-9391 [Print] China
• PMID: 14694588 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antidotes
• Cholinesterase Inhibitors
• Insecticides
• Pralidoxime Compounds
• Unithiol
• Cholinesterases
• pralidoxime

Topics

• Animals
• Antidotes (pharmacology)
• Cholinesterase Inhibitors (poisoning)
• Cholinesterases (blood)
• Dose-Response Relationship, Drug
• Insecticides (poisoning)
• Pralidoxime Compounds (pharmacology)
• Rats
• Unithiol (pharmacology)