Detection of von Willebrand factor-cleaving protease (ADAMTS-13) in human platelets.
|
| Abstract |
The hemostatic activity of von Willebrand factor (vWF) is strongly dependent on its multimeric structure, with the highest activity in 'unusually large' multimers secreted from endothelial cells. The multimeric structure is regulated by a plasma protease, vWF-cleaving protease (vWF-CP, or ADAMTS-13). ADAMTS-13 mRNA is variably expressed in liver and other tissues. Because 15-25% of circulating vWF is stored in platelets, the presence and function of ADAMTS-13 in platelets are important issues. Here we report ADAMTS-13 expression in human platelets. Western blot analysis and flow cytometric analysis on permeabilized platelets revealed the presence of ADAMTS-13 protein. Real-time PCR demonstrated that ADAMTS-13 mRNA is present in platelets of six healthy volunteers, with little quantitative difference. The presence of ADAMTS-13 in platelets may imply the functional role of this enzyme in the local regulation of platelet function at the site of vascular injury or thrombus formation, and provide a useful tool for the analysis of structure and function of this enzyme.
|
|---|---|
| Authors | Misako Suzuki, Mitsuru Murata, Yumiko Matsubara, Toshihiro Uchida, Hiroaki Ishihara, Toshiro Shibano, Shin-ichiro Ashida, Kenji Soejima, Yasunori Okada, Yasuo Ikeda |
| Journal | Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 313 Issue 1 Pg. 212-6 (Jan 02 2004) ISSN: 0006-291X [Print] United States |
| PMID | 14672719 (Publication Type: Journal Article) |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!