Immunohistochemical analysis of platelet-derived growth factor and its receptors in soft tissue malignant fibrous histiocytoma.

Abstract

BACKGROUND:

Little information is available regarding the expression of platelet-derived growth factor (PDGF) isoforms and their receptors in soft tissue malignant fibrous histiocytoma (MFH).

MATERIALS AND METHODS:

We investigated expression of PDGF isoforms and their receptors (PDGF-R alpha and -R beta) in 43 MFH tissue specimens using immunohistochemical techniques. Furthermore, we examined the correlation of PDGF expression in MFHs with proliferative activity assessed by MIB-1 immunohistochemical staining.

RESULTS:

Positive cytoplasmic immunoreactivity for PDGF-AA, -BB and -AB was identified in tumor cells of 28 (66%), 4 (10%) and 26 (61%), respectively, of the 43 MFHs analyzed. Positive cytoplasmic immunoreactivity for PDGF-R alpha and -R beta was identified in tumor cells of 41 (95%) and 32 (74%), respectively, of the MFHs. Thirty-four (79%) MFHs coexpressed one or more PDGF isoforms and their corresponding receptors. In PDGF-AA immunostaining, MIB-indices in the high immunoreactivity group (> 10% of tumor cells) were significantly higher than those in the low immunoreactivity group (< 10% of tumor cells) (p = 0.031).

CONCLUSION:

Our data provide evidence to support the presence of an autocrine/paracrine mechanism of proliferation control involving the PDGF ligand/receptor system in human MFHs.

• Authors: Tetsuji Yamamoto, Toshihiro Akisue, Takashi Marui, Ikuo Fujita, Keiji Matsumoto, Teruya Kawamoto, Toshiaki Hitora, Tetsuya Nakatani, Keiko Nagira, Masahiro Kurosaka
• Journal: Anticancer research (Anticancer Res) 2003 Sep-Oct Vol. 23 Issue 5b Pg. 4325-8 ISSN: 0250-7005 [Print] Greece
• PMID: 14666646 (Publication Type: Journal Article)

Chemical References

• Ki-67 Antigen
• Platelet-Derived Growth Factor
• Protein Isoforms
• Receptor, Platelet-Derived Growth Factor alpha
• Receptor, Platelet-Derived Growth Factor beta

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Cell Division (physiology)
• Female
• Histiocytoma, Benign Fibrous (metabolism, pathology)
• Humans
• Immunohistochemistry
• Ki-67 Antigen (metabolism)
• Male
• Middle Aged
• Platelet-Derived Growth Factor (biosynthesis)
• Protein Isoforms
• Receptor, Platelet-Derived Growth Factor alpha (biosynthesis)
• Receptor, Platelet-Derived Growth Factor beta (biosynthesis)