Abstract |
The distribution and functional characteristics of in vitro bone marrow (BM) endothelial colonies (CFU-En) were studied in 70 non-Hodgkin's lymphoma (NHL) patients in different phases of the disease to explore the association between CFU-En growth and angiogenesis, and between the number of CFU-En and the presence of hematopoietic and mesenchymal progenitor cells. The mean number of CFU-En/10(6) BM mononuclear cells seen in remission patients was significantly higher than that seen in newly diagnosed patients (P=0.04), and in normal subjects (P=0.008). Patients with low-grade NHL in remission displayed a higher CFU-En value compared with high-grade NHL (P=0.04). In the autograft group (40 patients), a significant reduction of CFU-En number was detected in the first 4-6 months after transplantation. In remission patients, the CFU-En number positively correlated with the incidence of BM colony-forming unit granulocyte-macrophage (CFU-GM) (P=0.013) and CFU-multilineage (CFU-GEMM) hematopoietic colonies (P=0.044). These in vitro data show that CFU-En numbers increase following standard-dose chemotherapy, thus providing a rationale for further investigating the effects of different cytostatic drugs on BM endothelial cells growth and function.
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Authors | F Lanza, D Campioni, M Punturieri, S Moretti, M Dabusti, R Spanedda, G Castoldi |
Journal | Bone marrow transplantation
(Bone Marrow Transplant)
Vol. 32
Issue 12
Pg. 1165-73
(Dec 2003)
ISSN: 0268-3369 [Print] England |
PMID | 14647271
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Antineoplastic Combined Chemotherapy Protocols
(therapeutic use)
- Biomarkers
- Bone Marrow Cells
(cytology)
- Cell Lineage
- Cells, Cultured
(cytology)
- Colony-Forming Units Assay
- Combined Modality Therapy
- Endothelial Cells
(cytology)
- Female
- Hematopoietic Stem Cells
(cytology)
- Humans
- Immunophenotyping
- Lymphoma, Non-Hodgkin
(drug therapy, pathology, therapy)
- Male
- Middle Aged
- Peripheral Blood Stem Cell Transplantation
- Remission Induction
- Transplantation, Autologous
- Transplantation, Homologous
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